AMINO ACID RECEPTORS IN NEONATAL TRIGEMINAL NEURONS

在发育过程中，哺乳动物表现出从哺乳到 为了摄入营养而进行的咀嚼行为。这些行为和 它们之间的过渡对于正常的发展和 生死存亡。构成神经电路的两个关键部件 负责口腔运动行为的是三叉神经运动神经元和 中脑三叉神经细胞。对口腔运动的洞察 通过对接码头的发展来获得发展 支配三叉神经运动神经元和中脑三叉神经神经元 随着神经递质受体复合体的开发利用 来调节这些神经元的活动。这项研究将是 第一个用化学物质表征口腔运动发育的方法 神经解剖学技术。这将通过关联来实现 三叉神经谷氨酸和氨基丁酸能神经支配的变化 运动神经元和三叉神经中脑神经元的个体发育 出生后早期这些神经元中的NMDA、AMPA和GABAA受体 发展。将执行三种不同的实验方案 在出生后第1天、第5天、第15天和第25天，因为这些年龄代表 口腔运动发育的关键时期。在第一组 实验中，抗谷氨酸的多克隆抗体和多克隆 谷氨酸脱羧酶(GAD)抗体将用于标记 三叉神经运动中的谷氨酸能和GABA能终末 核团和三叉神经中脑核。在第二组 实验，使用抗体的受体免疫组织化学 将使用NMDA、AMPA和GABAA亚基来描述时间 NMDA、AMPA和GABAA受体在三叉神经节的空间表达 运动神经元和三叉神经中脑神经元。最后， 使用[~3H]MK-801，[~3H]AMPA的定量受体放射自显影 [~3H]用蝇草酚测定NMDA、AMPA的浓度变化 和GABAA受体。这些研究的结果将 深入了解谷氨酸和GABA在控制 新生儿三叉神经运动神经元和中脑三叉神经神经元 活动，以进一步了解其发展和功能 早期口腔运动回路的特征。

During development mammals demonstrate a transition from suckling to chewing behavior for the ingestion of nutrients. These behaviors and the transition between them are essential for normal development and survival. Two critical components comprising the neural circuitry responsible for oral-motor behaviors are trigeminal motoneurons and mesencephalic trigeminal neurons. Insight regarding oral-motor development will be gained by correlating the development of terminals innervating trigeminal motoneurons and mesencephalic trigeminal neurons with the development of neurotransmitter receptor complexes utilized to modulate the activity of these neurons. This study will be the first to characterize oral-motor development using chemical neuroanatomical techniques. This will be accomplished by correlating changes in the glutamatergic and GABAergic innervation of trigeminal motoneurons and mesencephalic trigeminal neurons with the ontogeny of NMDA, AMPA, and GABAa receptors in these neurons during early postnatal development. Three different experimental protocols will be performed on rats at postnatal day 1, 5, 15, and 25 as these ages represent critical periods in oral-motor development. In the first set of experiments, a polyclonal antibody against glutamate and a polyclonal antibody against glutamate decarboxylase (GAD) will be used to label glutamatergic and GABAergic terminals throughout the trigeminal motor nucleus and mesencephalic trigeminal nucleus. In the second set of experiments, receptor immunohistochemistry using antibodies against NMDA, AMPA, and GABAa subunits will be used to describe the temporal and spatial expression of NMDA, AMPA, and GABAa receptors in trigeminal motoneurons and mesencephalic trigeminal neurons. Finally, quantitative receptor autoradiography using [3H]MK-801, [3H]AMPA, and [3H]muscimol will be used to determine density changes of NMDA, AMPA and GABAa receptors respectively. The results from these studies will provide insight into the role of glutamate and GABA in controlling neonatal trigeminal motoneuron and mesencephalic trigeminal neuron activity in order to further understand the development and function of early oral-motor circuits.