CLONING IRON RESPONSIVE GENES IN S MUTANS

这是一个修改后的，大大精简的，调查的建议， 铁在变形链球菌致龋能力中的作用。 如前所述，前提是铁在发病机制中起作用。 龋齿这一假设是基于大量的文献， 细菌病原体，在某些情况下， 为了获得铁。此外，已知铁 获得与已建立的毒力机制直接相关。在 修改后，作者将继续论证铁在其中发挥作用， 在龋齿的病因学，通过建立以下机制： 其中S.变形杆菌获得铁;铁敏感性的鉴定 S.以及铁的克隆和表征 生物体中的应答基因。未来的实验将在 以前的目的涉及铁应答基因和大鼠的诱变， 龋齿实验以验证基因在龋齿发生中的作用;以及， 最后进行RNA和基因融合实验， 应答基因受到转录调控。

This is a revised, and greatly streamlined, proposal to investigate the role that iron may play in the cariogenic ability of Streptococcus mutans. The premise, as before, is that irons plays a role in pathogenesis of caries. The hypothesis is based on the large literature for other bacterial pathogens which contains, in some cases, elaborate mechanisms for the acquisition of iron. Furthermore, it is known that iron acquisition is directly tied in with established virulence mechanisms. In the revision, the author will pursue the argument that iron plays a role in the etiology of dental caries by establishing the following: mechanisms by which S. mutans acquires iron; identification of iron-responsive proteins in S. mutans; and the cloning and characterization of iron responsive gene(s) in the organism. Future experiments will hold forth on the previous aims concerning mutagenesis of iron responsive genes and rat caries experiments to verify the role of the genes in cariogenesis; and, finally RNA and gene fusion experiments to determine whether iron responsive genes are transcriptionally regulated.