RETINAL NEUROTRANSMITTERS AND SYNAPTIC CIRCUITRY

描述(改编自申请者的摘要)：建议的作品将使用 免疫细胞化学技术相结合的方法来表征肿瘤部位 受体对谷氨酸的作用及其相互关系 视网膜内的兴奋性神经递质。具体目标是：(1) GluR亚基定位到细胞和亚细胞位置，使用 用光镜和电子显微镜免疫细胞化学方法鉴定血管内皮细胞 猫视网膜表达AMPA、海人藻酸、NMDA和 Glur的代谢性类型及其相互关系 突触特化的受体；(2)受体的比较 不同亚群间突触关系的分布 视网膜神经元，使用双标记技术比较分布 GluRs与各种神经化学标记物的关系 神经递质、合成酶、钙结合蛋白和第二 信使，特别关注这些关系是如何 与特定细胞的生理特性相关；以及(3) 钙结合蛋白和钙结合蛋白的Glur分布比较 依赖于钙离子的第二信使，使用双标记组织来确定 视网膜的定位是否与 调控或执行中的钙结合蛋白和第二信使 GluR亚基的功能。

DESCRIPTION (Adapted from applicant's abstract): The proposed work will use a combination of immunocytochemical techniques to characterize the sites of action and relationships of receptors responsive to glutamate, the major excitatory neurotransmitter within the retina. The specific aims are: (1) the localization of GluR subunits to cellular and subcellular sites, using light and electron microscopic immunocytochemistry to identify the cells in the cat retina that express subunits of the AMPA, kainate, NMDA and metabotropic types of GluR and to determine the relationship of these receptors to synaptic specializations; (2) comparison of the receptor distribution with the synaptic relationships of defined subpopulations of retinal neurons, using double-label techniques to compare the distribution of GluRs with a variety of neurochemical markers including neurotransmitters, synthetic enzymes, Ca2+-binding proteins, and second messengers, with specific attention being given to how these relationships correlate with the physiological properties of specific cells; and (3) comparison of GluR distribution with Ca2+-binding proteins and Ca2+-dependent second messengers, using double-labeled tissues to determine whether the retinal localizations are consistent with a role for Ca2+-binding proteins and second messengers in regulation or implementation of the functions of GluR subunits.