ASSEMBLY AND PLACEMENT OF THE CELL DIVISION RING

描述：这位研究者发现了一个新基因cdc12。 在组装和放置中似乎起着重要作用的庞贝 在收缩环上。他假设在间期，cdc12 存在于一个大型的多蛋白复合体中，它沿着微管传播到 细胞的中央，用来标记未来的裂沟位置。 在有丝分裂过程中，假定cdc12蛋白参与肌动蛋白。 通过与肌动蛋白结合蛋白的相互作用进行环组装 侧写。张博士的近期目标是检验这些假说。三 提出了具体目标：(1)确定政府职能 Cdc12和cdc3之间的相互作用(Profilin)；(2)识别和 鉴定与cdc12p相互作用的其他蛋白质；和(3) 通过检查环来研究环形成的空间方面 在活细胞中形成。

DESCRIPTION: This investigator has identified a novel gene, cdc12, in S. pombe that appears to play an important role in the assembly and placement of the contractile ring. He hypothesizes that during interphase, cdc12 exists in a large multiprotein complex that travels along microtubules to the middle of the cell to mark the future site of the cleavage furrow. During mitosis, the cdc12 protein is hypothesized to participate in actin ring assembly through an interaction with the actin-binding protein profilin. Dr. Chang's immediate goal is to test these hypotheses. Three specific aims are proposed: (1) to determine the function of the interaction between cdc12 and cdc3 (profilin); (2) to identify and characterize additional proteins that interact with cdc12p; and (3) to investigate the spatial aspects of ring formation by examining ring formation in living cells.