CORE--LABORATORY

实验室核心提供人员、专业知识和方法， 测量和定量各种介质，蛋白质和细胞， 是IDCRC目标和项目的核心。的聚集 将这些技术应用于中心设施， 控制，数据检索和数据分析将由几个 在必要领域具有既定专门知识的个人， IDCRC项目。大多数方法都在核心 研究人员的实验室。具体而言，两种组胺方法是 LTC 4和PGD 2的RIA也可用。测定血清白蛋白、激肽和 纤维蛋白原是公认的，肺和鼻的方法也是如此。 液体的回收和分析以及 细胞内流核心研究者有特异性血清IgE测定 和IgG抗体，并具有新开发的用于IgM、伊加和sIgA的ELISA。 测量几种前列腺素（例如，PGD2，9 α-11 β PGF2， PGE 2等）质谱法（GCMS）。其他GCMS IDCR项目所需的技术已经开发出来， 核心研究者测量LTB 4、尿甲基组胺和 可的松/皮质醇在适当的位置。核心研究人员已经开发出 几种细胞因子的“内部”测定，包括白细胞介素4、5、6、8 和INF-γ。商业测定通常用于其他细胞因子 必要时RT-PCR技术也已到位，并将用于 分析细胞流入和活化。

The Laboratory Core provides the personnel, expertise and methodologies to measure and quantitate the various mediators, proteins and cells which are, central to the goals and projects in the IDCRC. The aggregation of these technologies to a central facility ensures that assay quality control, data retrieval and data analysis will be done by several individuals with established expertise in the areas necessary for the IDCRC projects. Most of the methodologies are in place in the Core investigators' laboratories. Specifically, two histamine methods are available as are RIAs for LTC4 and PGD2. Assays, serum albumin, kinins and fibrinogen are well-established, as are methodologies for lung and nasal challenges with retrieval and analysis of fluids and quantitation of cellular influx. The Core investigators have assays for specific serum IgE and IgG antibodies, and have newly developed ELISAs for IgM, IgA and sIgA. Measurements for several prostanoids (e.g., PGD2, 9alpha-11beta PGF2, PGE2, etc.) by mass spectrometry (GCMS) are established. Other GCMS technologies necessary for projects in the IDCR have been developed by Core investigators to measure LTB4, urinary methylhistamine, and cortisone/cortisol are,in place. The Core investigators have developed "in-house" assays for several cytokines, including interleukins 4,5,6,8 and INF-gamma. Commercial assays are routinely used for other cytokines when necessary. RT-PCR technology is also in place and will be used in analysis of cellular influx and activation.