HIV 1 VPR INTERACTION WITH A HOST CELLULAR PROTEIN

描述（改编自研究者摘要）：中心焦点 本申请的一个目的是克隆Vpr相互作用蛋白的cDNA (RIP)探讨Vpr功能的生化机制， HIV复制过程中的RIP。 具体而言，RIP将从 细胞提取物和微测序，因此寡核苷酸探针可以 设计用于筛选cDNA文库。 然后，RIP表达和激活 将检查不同类型的原代人血细胞。 随后，VPR和RIP在HIV复制过程中的作用将被 通过建立Vpr或RIP表达细胞系， 用反义核酸抑制内源性RIP表达 寡核苷酸，并通过使用体内以及体外系统， 检测Vpr和RIP对HIV早期转录的影响 前病毒 从这些研究中，作者希望获得以下信息： 辅助基因如何调节宿主中的HIV复制 vpr和蜂窝目标RIP。

DESCRIPTION (Adapted from investigator's abstract): The central focus of this application is to clone the cDNA for the Vpr-interacting protein (RIP) and to explore the biochemical mechanism of function of Vpr and RIP during HIV replication. Specifically, RIP will be purified from cellular extracts and micro-sequenced so oligonucleotide probes can be designed to screen cDNA libraries. Then, RIP expression and activation in different types of primary human blood cells will be examined. Subsequently, the role of VPR and RIP during HIV replication will be examined by establishing Vpr or RIP-expressing cell lines, by suppression of endogenous RIP expression with anti-sense oligonucleotides, and by using in vivo as well as in vitro systems to measure effects of Vpr and RIP on the early transcription of the HIV provirus. From these studies the author expects to gain information on how HIV replication in the host may be regulated by the accessory gene vpr and the cellular target RIP.