METABOLISM OF HDL SUBPOPULATIONS--INFLUENCE OF DIET

描述：已知高密度脂蛋白浓度为 在评估冠心病风险方面很重要。饮食上的减少 众所周知，脂肪可以降低高密度脂蛋白浓度，但对此知之甚少 对高密度脂蛋白亚组分分布的影响。在某些高脂血症中 状态，如高甘油三酯血症，高密度脂蛋白浓度较低。因此， 这一应用的核心问题之一是饮食是否会导致 高密度脂蛋白的降低将导致比较的高密度脂蛋白亚组分发生类似的变化 对于因高脂血症而导致高密度脂蛋白过低的受试者。在…的第一部分 正常血脂和高脂血症受试者的高密度脂蛋白亚组分 将被研究和饮食诱导的高密度脂蛋白变化的作用及其 将评估与高密度脂蛋白亚组分变化的关系。在 第二部分建议，调制高密度脂蛋白的分布 将研究高密度脂蛋白的亚组分和形成机制。 培养中的成纤维细胞和巨噬细胞。这些后来的研究将需要 双向定量电泳法分离高密度脂蛋白的优势 未经离心分离的亚组分。

DESCRIPTION: High density lipoprotein concentrations are known to be important in assessing coronary heart disease risk. Reduction in dietary fat is known to decrease HDL concentrations, but little was known regarding its influence on HDL subfraction distribution. In certain hyperlipidemic states, such as hypertriglyceridemia, HDL concentrations are lower. Thus, one of the central questions of this application is whether diet-induced reduction in HDL will result in similar changes in HDL subfractions compared to subjects who have low HDL due to hyperlipidemia. In the first part of the proposal, HDL subfractions in normolipidemic and hyperlipidemic subjects will be studied and the role of diet-induced HDL changes and their relationship to changes in HDL subfractions will be evaluated. In the second part of the proposal, modulation of the distribution of HDL subfractions and mechanism of HDL formation will be studied using fibroblasts and macrophages in culture. These later studies will take advantage of two-dimensional quantitative electrophoresis to isolate HDL subfractions without centrifugation.