INFECTIVITY OF PAUCIBACILLARY PULMONARY TUBERCULOSIS

描述 FIRCA应用程序的父项目是分子流行病学 墨西哥恰帕斯州结核分枝杆菌的研究。这个 调查人员正在使用分子基因分型方法来描述 所有抗酸杆菌涂片中结核分枝杆菌的多样性 肺结核阳性病例。群集性结核病患者感染了 同样的菌株将在人口、地理和 社会经济学术语。选定的生物、社会和行为因素 群集性病例将与非群集性病例进行比较。结核病的成立 分子流行病学实验室(DNA指纹、聚合酶链式反应和快速药物 敏感度测定)在国家诊断研究所 墨西哥城的流行病是一个技术转移目标。 FIRCA研究的目的是应用分子流行病学 技术(分离株的IS6110基因分型)评估患有 痰镜检不出微生物确诊的肺结核 (少杆菌)确实是其他人的感染源。这将会 允许重新评估只有AFB涂片阳性病例的假设 有助于疾病的传播。由此产生的分子基因型将 进行分析以识别结核病例聚集性，然后进行关联 在通过流行病学方法获得的病例中发生传播事件。 这项研究将在墨西哥普埃布拉州进行，该州 大部分是农村地区，在社会和经济上处于边缘。

DESCRIPTION The parent project for this FIRCA application is a molecular epidemiologic study of Mycobacterium tuberculosis in the state of Chiapas, Mexico. The investigators are using molecular genotyping methods to describe the diversity of M. tuberculosis strains from all acid-fast bacilli (AFB) smear positive cases of pulmonary TB. Clusters of TB patients infected with the same strain will be characterized in demographic, geographic, and socioeconomic terms. Biologic, social, and behavioral factors of selected clusters will be compared to non-clustered cases. The establishment of a TB molecular epidemiology lab (DNA fingerprinting, PCR and rapid drug susceptibility determination) in the Instituto Nacional de Diagnostico y Epidemiologicas in Mexico City is a technology transfer goal. The purpose of the FIRCA study is to apply molecular epidemiologic techniques (IS6110 genotyping of isolates) to evaluate whether persons with microbiologically confirmed pulmonary TB not detected by sputum microscopy (paucibacillary) truly are a source of infection to others. This would enable reassessment of the assumption that only AFB smear positive cases contribute to disease transmission. The resulting molecular genotypes will be analyzed to identify clusters of TB cases and will then be correlated with transmission events in the cases obtained by epidemiologic methods. This study will be conducted in the state of Puebla, Mexico, which is largely rural, and socially and economically marginal.