TRYPTOPHAN DEPLETION AND CSF MONOAMINE METABOLITES

血浆色氨酸（TRP）消耗越来越多地被许多人使用， 研究人员作为确定大脑作用的研究工具 5-羟色胺（5-HT）在各种精神疾病中起作用， 它的简单性，安全性和强大的行为效果， 一些患者群体。 然而，迄今为止没有任何研究表明， 这项技术实际上改变了人类大脑中的5-HT代谢物。 本研究 将测定脑脊液（CSF）单胺代谢物 在12例缓解的抑郁症患者中， 无酒精，无药物至少3年 12名年龄性别匹配的健康对照者。 预计 缓解的抑郁症患者会经历抑郁症的复发， TRP消耗期间的症状，但不是健康对照。 后 初步筛选、知情同意和基线评估，12 符合DSM-IV既往重度抑郁发作标准的患者， 且HAM-D评分≥ 9分，年龄性别匹配者12例 没有精神障碍的个人或家族史的受试者将 进行测试。 将进行TRP耗竭和对照检测 采用安慰剂对照、双盲、随机、交叉设计。 这包括两个为期三天的测试，间隔一周。 在一次测试中， 将接受103克，无TRP，15氨基酸饮料（TRP耗尽）， 在另一个测试中，一种105克，补充TRP的16种氨基酸饮料 （对照）。 血浆游离和总TRP水平，大分子中性氨基酸 水平和行为评级将在五小时前获得 在摄入每种氨基酸饮料后。 其主要目的是 一个项目是确定TRP耗竭是否会导致CSF 5-HIAA减少 而不是HVA和MHPG，并确定TRP的血浆测量值 消耗与CSF 5-HIAA最相关。 此外，该项目 将使我们能够获得有关变化关系的初步数据， 在TRP耗竭至CSF 5-HIAA期间的情绪。 本研究将提供 解释血浆TRP相关性的基本数据 5-HT的功能。 有关的初步数据 CSF 5-HIAA与对TRP耗竭的抑郁反应的关系将 作为莫雷诺博士未来调查的基础

Plasma tryptophan (TRP) depletion is increasingly being used by many investigators as a research tool for determining the role of brain serotonin (5-HT) function in a variety of psychiatric disorders because of its simplicity, safety, and robust behavioral effects observed in some patient groups. However no study to date has shown that this technique actually alters brain 5-HT metabolites in humans. This study will determine the cerebrospinal fluid (CSF) monoamine metabolite response to TRP depletion in 12 remitted depressed patients who have been symptom-free, and medication-free for a period of at least 3 months, and 12 age-gender matched healthy controls. It is anticipated that remitted depressed subjects will experience a relapse of depressive symptoms during TRP depletion, but not the healthy controls. After initial screening, informed consent, and baseline assessment, 12 patients meeting DSM-IV criteria for a past major depressive episode, and HAM-D score greater than or equal to 9, and 12 age-gender matched subjects without personal or family history of mental disorders will undergo testing. TRP depletion and control testing will be administered in a placebo-controlled, double-blind, randomized, cross-over design. This includes 2 three-day tests 1 week apart. On one test the subjects will receive 103 gm, TRP-free, 15-amino acid drink (TRP depletion) and on the other test a 105 gm, TRP-supplemented 16-amino acid drink (control). Plasma free and total TRP levels, large neutral amino acid levels, and behavioral ratings will be obtained prior to and five hours after ingestion of each amino acid drink. The primary aim of this project is to determine if TRP depletion causes a decrease of CSF 5-HIAA but not in HVA and MHPG, and to determine which plasma measure of TRP depletion best correlate with CSF 5-HIAA. Additionally this project will allow us to obtain preliminary data on the relationship of change in mood during TRP depletion to CSF 5-HIAA. This study will provide essential data on which to interpret the relevance of plasma TRP depletion to 5-HT function in humans. Preliminary data regarding the relationship of CSF 5-HIAA to depressive response to TRP depletion will serve as a basis for future investigations by Dr. Moreno.