IN SITU PREPARATION--POLYMER STATIONARY PHASES FOR HPLC

拟议研究的总体目标是制定程序 用于原位制备高效的聚合物填充柱 高效液相色谱分析。在第一阶段， 芬里斯技术研究公司已经确定了 它的方法是开发出具有可比性的 对广泛使用的商品聚合物哈密顿PRP-1的效率 柱子直径为5微米。最低要求 费尔尼斯塔的折合塔板高度小于 PRP-1。此外，在校正静止状态下的差异之后 相颗粒直径、Fenris柱反压 比汉密尔顿PRP-1低五倍以上。 在第二阶段，Fenris Technology Research，Inc.建议进一步 探讨配方和聚合调理的效果 关于固定相性能，目标是进一步 提高效率。固定相的特征是 测定层析效率，检查形态 扫描电子显微镜和测量表面积和 孔隙率由BET氮气吸附方法测定。此外，在 将开发现场程序以引入离子官能团 将基团连接到聚苯乙烯主链上，从而使 离子电池可实现低背压、高效率 交换层析和反相层析。 建议的商业应用 该项目将导致低成本，高效的聚合物色谱柱 分析型和制备型高效液体 层析法。

The overall goal of the proposed research is to develop procedures for in situ preparation of efficient polymer packed columns for high performance liquid chromatography (HPLC). In Phase I, Fenris Technology Research, Inc., has established the feasibility of its approach by devoloping formulations that are comparable in efficiency to Hamilton PRP-1, a widely used commercial polymer column with five micron diameter particles. The minimum reduced plate height for the Fernis column is smaller than for PRP-1. Furthermore, after correcting for difference in stationary phase particle diameter, the backpressure with the Fenris column is more than five times lower that with the Hamilton PRP-1. In phase II, Fenris Technology Research, Inc., proposes to further explore the effect of formulation and polymerization conditioning on stationary phase performance with the objective of further improving efficiency. Stationary phases will be characterized by determining chromatographic efficiency, examining morphology by scanning electron microscopy and measuring surface area and porosity by the BET nitrogen adsorption method. In addition, in situ procedures will be developed for introducing ionic functional groups onto the polystyrene backbone so that the advantages of high efficiency with low backpressure can be realized with ion exchange as well as reversed phase chromatography. PROPOSED COMMERCIAL APPLICATION This project will lead to low cost, effecient polymer columns for both analytical and preparative high performance liquid chromatography.