PROTEIN/WATER COHERENCE EFFECTS IN NMR AND IMAGING

说明：这是一份经修订的续设提案。 各项广泛目标 是开发梯度和射频脉冲整形的应用 用于生物分子的核共振光谱，特别是在 浓缩溶剂。 重点是分子间多量子 (iMQ)蛋白质和水之间的相互作用和交叉作用。 两者 理论和实验工作将继续在稀溶液的 水中的生物分子 具体来说，体内成像和光谱学 工作将扩大，目的是提高图像对比度， 验证功能性MRI的机制。 高分辨率NMR 将使用产生不均匀场磁体来追求更高的场 (e.g. NHMFL现有的27 T和拟议的30 T磁体）。 工作 通过溶质-溶剂相干转移小信号放大也将 追求的观点是彻底改变低伽马或 在NMR中检测到低浓度物质。 iMQ的抑制 将探索诸如TOCSY的粘性蛋白序列以产生改进的 版本.

DESCRIPTION: This is a revised continuation proposal. The broad objectives are to develop applications of gradients and radiofrequency pulse shaping for nuclear resonance spectroscopy of biomolecules, particularly in concentrated solvents. The focus is on intermolecular multiple-quantum (iMQ) coherences and crosspeaks between proteins and water. Both the theoretical and experimental work will be continued on dilute solution of biomolecules in water. Specifically, the in vivo imaging and spectroscopy work will be extended with the aims of enhancing image contrast and validating proposed mechanisms for functional MRI. High resolution NMR at high fields will be pursued using magnets which give inhomogeneous field (e.g. the existing 27T and proposed 30T magnets at the NHMFL). Work on small-signal amplification by solute-solvent coherence transfer will also be pursued with the view of revolutionizing the way in which low-gamma or low-concentration species are detected in NMR. Suppression of iMQ coherences in sequences such TOCSY will be explored to produce improved versions.