DIETARY FAT AND THE OXIDATION OF PLAQUE LIPIDS

描述：拟议的研究将调查脂肪酸是否 类型和维生素E浓度调节：(1)脂类的数量 从动脉粥样硬化斑块中分离出的氧化产物，以及(2) 导致脂质氧化产物形成的机制。NZW 兔子将被维持在富含饱和、单不饱和的饲料中 或n-6多不饱和脂肪。这些饮食将包含高或低的成分 维生素E的浓度GC-MS将用于表征脂质 氧化产物，并推断导致氧化的机理 假设油酸、3-硝基酪氨酸的单过氧化产物 和3-氯酪氨酸反映过氧亚硝酸根的氧化反应， 分别是自由过渡金属或髓过氧化物酶。

DESCRIPTION: The proposed studies will investigate whether fatty acid type and vitamin E concentrations modulate: (1) the amounts of lipid oxidation products isolated from atherosclerotic plaques, and (2) the mechanisms that lead to the formation of lipid oxidation products. NZW rabbits will be maintained on diets enriched in saturated, monounsaturated or n-6 polyunsaturated fat. The diets will contain either high or low concentrations of vitamin E. GC-MS will be used to characterize lipid oxidation products and to infer the mechanisms leading to oxidation assuming that mono-peroxidation products of oleic acid, 3-nitrotyrosine and 3-chlorotyrosine reflect initiation of oxidation by peroxynitrite, free transition metals or Myeloperoxidase, respectively.