NMR INVESTIGATION OF HUMAN CD58 CELL SURFACE RECEPTOR

这项研究的目的是了解基本原则 参与细胞表面受体之间的相互作用。建议数 研究人员将研究这种相互作用的机制和特殊性 在人类白细胞CD2和CD58细胞表面黏附受体之间， 使用现代核磁共振波谱和基因工程。人白细胞 表面受体负责许多生物过程，如 细胞黏附、细胞信号和细胞识别，这些在 发展、免疫反应、肿瘤转移和伤口愈合。我们的 初步研究将集中在核磁共振结构的确定上 人CD58受体的粘附域。人CD2和CD2基因突变的研究 CD58受体将被用来研究结合部位和结合 CD2/CD58相互作用的特异性以及核磁共振结构 CD2和CD58粘附域之间复合体的测定。 这项研究将增加我们对免疫学和细胞学的理解 生物学，并有可能导致发现可用于 影响细胞表面特异性结合活性的疾病防治 感受器。

The purpose of this research is to learn about the basic principles involved in the interactions among cell surface receptors. The proposed research will study the mechanism and specificity of the interaction between the human leukocyte CD2 and CD58 cell surface adhesion receptors, using modern NMR spectroscopy and genetic engineering. Human leukocyte cell surface receptors are responsible for many biological processes such as cell adhesion, cell signaling and cell recognition, which are important in development, immune responses, tumor metastasis and wound healing. Our initial research will focus on the NMR structure determination of the adhesion domain of human CD58 receptor. Mutational studies of human CD2 and CD58 receptors will be carried out to study the binding site and binding specificity of the CD2/CD58 interactions, along with the NMR structure determination of the complex between the adhesion domains of CD2 and CD58. This research study will increase our understandings in immunology and cell biology, and lead potentially to the discovery of drugs that can be used in disease control by affecting specific binding activities of cell surface receptors.