LITHIUM INCREASES DOPAMINERGIC PHENOTYPE IN HNT NEURONS

描述：（改编自摘要）：这是第一次I期 应用于研究暴露的hNT神经元的稳定性和活力 增加DA型表型。 hNT神经元是有丝分裂后终末分化的人神经元，其可以 用于帕金森氏症等疾病的移植手术。这些 神经元可以表达DA，这使它们成为胎儿组织的可行替代品， 然而，在这种植入物中，这些神经元中DA的表达率并不高。 暴露于LiCl可显著增加该速率。这项建议会 确定TH的表达增加是否指示真的 功能性DA表型以及该表型是否继续在 PD的动物模型。 拟定商业应用： 如果这是事实，那么我们将有可能有一个高多巴胺能 一种大量可用的细胞，可以代替胎儿组织进行神经 帕金森病的移植治疗

DESCRIPTION: (adapted from abstract): This is a first time Phase I application to investigate the stability and viability of hNT neurons exposed to lithium to increase DA-type phenotype. hNT neurons are postmitotic, terminally differentiated human neurons which can be used in transplant procedures for such diseases as Parkinson's. These neurons can express DA which make them a viable alternative to fetal tissue for such implants, however, the rate of DA expression is not high in these neurons. The rate can be increased significantly by exposure to LiCl. This proposal will determine whether the increased expression of TH is indicative of true functional DA phenotype and whether the phenotype continues to be expressed in an animal model of PD. PROPOSED COMMERCIAL APPLICATION: If this is shown to be the case then we will potentially have a highly dopaminergic cell that is available in large quantities that can replace fetal tissue for neural transplantation treatments in Parkinson's disease.