IDENTIFICATION OF BACTERIA DIRECT FROM BLOOD CULTURE

直接从血液培养瓶中快速识别细菌将加快患者的治疗和恢复，并大大降低医院护理成本。传代培养目前的程序通常需要24至48小时才能获得细菌鉴定。气相色谱法分析细菌脂肪酸有希望成为一种快速和廉价的鉴定技术，如果该系统的灵敏度更高，如果可以最大限度地减少血液中脂肪酸组成的影响。整个脂肪酸萃取物的无分裂注射和电子捕获检测器的使用可将色谱灵敏度提高约600万倍。将研究通过梯度离心和/或过滤从血液成分中物理分离细菌，以及使用软件技术来减少血液脂肪酸成分的影响。将评估命名细菌和处理多微生物培养的新算法。通过比现有技术更快地进行鉴定，该技术可以在进行血液培养的情况下，为每个病人每天节省大约1000美元的护理费用。通过加速治疗和恢复，这项技术还将减少病人的痛苦，并有助于减少医院感染。建议的商业应用：直接从血液培养瓶中鉴定细菌将确保败血症患者得到快速治疗，从而减少医疗费用，从而至少节省一天的时间。MIDI将销售与该应用相关的软件、气相色谱仪和耗材，并将基于该技术开发其他应用。

Rapid identification of bacteria direct from a blood culture bottle would speed patient treatment and recovery and greatly reduce hospital care costs. The current procedure of subculturing typically takes from 24 to 48 hours to obtain bacterial identifications. Gas chromatographic analysis of bacterial fatty acids has promise as a rapid and inexpensive identification technique if the system were made more sensitive and if impact of the fatty acid composition of the blood could be minimized. Splitless injection of the entire fatty acid extract and use of an electron capture detector may increase the chromatographic sensitivity by a factor of ca. 6,000,000X. Physical separation of bacteria from blood components by gradient centrifugation and/or filtration and the use of software techniques for reducing the impact of blood fatty acid components will be researched. New algorithms for naming bacteria and for dealing with polymicrobic cultures will be evaluated. By producing identification more rapidly than current techniques, the proposed technique could save ca. $1,000 per patient day of care costs where blood cultures are taken. By speeding treatment and recovery, this technique will also reduce patient suffering and aid in reducing nosocomial infections. PROPOSED COMMERCIAL APPLICATIONS: Identification of bacteria direct from a blood culture bottle will save at least one day by assuring speedy treatment of patients with septicemia thereby reducing medical care costs. MIDI will sell the software, gas chromatograph and consumables related to this application and will develop other applications based on the technique.