TWO DIMENSIONAL INTEGRAL ELECTROPHORESIS GEL

描述：(改编自申请人的摘要)自推出以来 20年前，双向(2D)聚丙烯酰胺凝胶电泳法 仍然是最强大、利用率最低的净化方法 鉴定和结构鉴定所需的蛋白质数量 人物刻画。尽管人们几乎可以想象1D的任何应用 SDS PAGE将受益于近两个数量级的增长 由第二维度提供的分辨率，很少有实验室常规使用 双向凝胶电泳法。原因是用于生产的技术和设备 执行这一程序是如此繁琐，并且需要如此高水平的 大多数科学家不愿尝试掌握的专业知识 这项技术。在广泛的阵列上释放2D凝胶电泳的力量 对于生化和生物医学问题，我们设计了一个预制的、完整的2D 凝胶系统，其特点是易于使用。随着后基因组的出现 流感嗜血杆菌和其他12种微生物的时代 在人类方面取得的进展比其他50个基因组都要快， 利用双向凝胶电泳法了解蛋白质组的机会 人类疾病从未像现在这样严重，如果这项申请得到资助， 将永远不会更接近。 建议的商业应用：不可用

DESCRIPTION: (adapted from applicant's abstract) Since its introduction over twenty years ago, Two dimensional (2D) polyacrylamide gel electrophoresis remains the most powerful and most under utilized approach to purifying the quantities of proteins needed for identification and structural characterization. Although virtually any application one could imagine for 1D SDS PAGE would benefit from the nearly two orders of magnitude increase in resolution provided by the second dimension, few laboratories routinely utilize 2D gel electrophoresis. The reason is that techniques and equipment for carrying out this procedure are so cumbersome and require such a high level of expertise that the majority of scientists are dissuaded from trying to master this technique. To unleash the power of 2D gel electrophoresis on a wide array of biochemical and biomedical problems, we have designed a precast, integral 2D gel system whose hallmark is ease of use. With the advent of the post genome era for Haemophilus Influenzae and twelve other micro-organisms and with the rapid progress being made on the human and greater than fifty other genomes, the opportunity for utilizing 2D gel electrophoresis to understand the proteome and human disease have never been greater and, if this application is funded, will never have been within closer reach. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE