NOVEL NON-RADIOACTIVE NUCLEIC ACID LABELING REAGENTS

简单且可重复性地生产高标记的核的能力 酸性探针是大分子基团的一个重要方面 涉及杂交的生物学和DNA诊断应用 反应。然而，到目前为止，还没有一种简单的方法来生产高度 带有标签的探头，适用于广泛的应用。我们有 采用合理的方法设计了一系列非放射性标记 应该可以缓解目前的许多缺点的试剂 使用标签协议。这些新的标记试剂旨在 共价连接四种常见的非放射性检测方法中的任何一种 任何核酸的标记。建议的标签程序如下： 步骤，有效，并且应该产生稳定的标记核酸 从长远来看。鉴于它们的易用性和高效率，标签 将在各种分子和细胞中发挥巨大的作用 生物研究，并具有巨大的商业潜力。 建议的商业应用： 这种非放射性核酸标记的新方法将是 商业化者： (1)将该技术以试剂盒的形式出售给生物医学和 基础研究人员 (2)将技术授权给制药公司进行诊断 目的。

The ability to simply and reproducibly produce a highly labeled nucleic acid probe is a very important aspect for the large group of molecular biology and DNA diagnostic applications that involve a hybridization reaction. Yet to date, there is not an easy way to produce a highly labeled probe that is optimal for a wide range of applications. We have used a rational approach to design a series of non-radioactive labeling reagents that should alleviate many of the shortcomings of the currently used labeling protocols. These new labeling reagents are designed to covalently attach any one of four common non-radioactive detectable labels to any nucleic acid. The proposed labeling procedures are one- step, efficient, and should create labeled nucleic acids that are stable long term. Given their ease of use and high efficiency, the labeling will be of great utility in a wide variety of molecular and cellular biological studies and have huge commercial potential. PROPOSED COMMERCIAL APPLICATION: This new method of non-radioactive nucleic acid labeling will be commercialized by: (1) selling the technology in the form of reagent kits to biomedical and basic researchers (2) licensing the technology to pharmaceutical companies for diagnostic purposes.