NEW POLYMER PHASES FOR EFFICIENT HPLC

该项目的目标是制备新的聚合物固定相 高效液相色谱比聚苯乙烯更有效的层析- 二乙烯基苯。该方法将合成一种由以下组成聚合物 主要由交替的亲水和疏水单元组成。这个 这些单位的结构及其相对浓度将是 调整以生产具有类似膨胀趋势的材料 无论是极性溶剂还是非极性溶剂。因此，这种材料可以 多孔性，不会随着流动相的变化而膨胀或收缩 组成。它不需要像以前那样高度交联和刚性 聚苯乙烯-二乙烯基苯。这将改善色谱学 效率，因为通过聚合物相的传质速度 将比使用聚苯乙烯-二乙烯基苯更快。具体目标 第一阶段的目的是通过以下方式证明这一方法的可行性 制备一种聚合物固定相，该固定相产生4个还原板 或更少，同时保持与商业上类似的稳定性和容量 可用列。在第二阶段，层析特征 我们将进一步探讨这些阶段。 建议的商业应用 反相高效液相色谱柱的全球市场有几百个。 百万美元。这个项目的成功将使Fenris Technology Research，Inc.，准备在这一市场中占据相当大的份额 市场。

The goal of this project is to prepare new polymer stationary phases for HPLC that yield more efficient chromatography than polystyrene- divinylbenzene. The approach will be synthesize a polymer consisting predominantly of alternating hydrophilic and hydrophobic units. The structures of these units and their relative concentrations will be adjusted to produce a material that has similar swelling tendencies in both polar and nonpolar solvents. Therefore, this material can be porous without tending to swell or shrink with changing mobile phase composition. It will not have to be as highly crosslinked and rigid as polystyrene-divinylbenzene. This will improve chromatographic efficiency because the rate of mass transfer through the polymer phase will be faster thatn with polystyrene-divinylbenzene. The specific aim of Phase I is to demonstrate the feasibility of this approach by preparing a polymer stationary phase that yields a reduced plate of 4 or less while retaining stability and capacity similar to commercially available columns. In Phase II, the chromatographic characteristics of these phases will be further explored. PROPOSED COMMERCIAL APPLICATION The worldwide market for reversed phase HPLC columns is several hundred million dollars. Success in this project would put Fenris Technology Research, Inc., in position to capture a significant share of this market.