TARGETED SLOW RELEASE ANTICARIES AGENT

本提案的目的是设计和测试体外和 针对两种主要致龋细菌-- 变形链球菌和乳酸杆菌。目标是学习和 获得缓释抗菌剂的最佳功能， 具有足够的杀菌效力和低毒性的组分。这 与受体特异性蛋白质连接在一起， 致龋微生物。两个主要的受体候选者已经被 鉴定一种是凝集素样蛋白， 另一种是在牙本质中发现的蛋白质， 牙根，其减轻了细菌属的结合。在这 locale.受体特异性蛋白质使联合剂具备1） 手术般精确的特异性， 细菌和2）增加的致命操作的停留时间， 自杀式的联系一种“鸡尾酒”组合的杀真菌剂-受体特异性 根据概述的结果按比例混合配体 旨在激发代理人能力的实验：目标 或仅与致龋细菌精确结合; 作用部位暴露期，抑制细菌糖酵解 在固体支持物上生长;并杀死居住在牙齿上的细菌 羟基磷灰石 拟定商业应用： 漱口水市场规模为20亿美元。除了一个处方 品牌，没有一个是有效的预防或减少风险的牙科 龋齿

The purpose of this proposal is to design and test in vitro and effective anticaries agent which target two major cariogenic bacteria-- Streptococcus mutants and lactobacilli spp.. The goal is to study and obtain the optimum functioning of a slowly-releasing antibacterial component having adequate bactericidal potency and low toxicity. This is linked together with receptor-specific proteins which target the cariogenic microorganisms. Two prime receptor candidates have been identified. One is a lectin-like protein, shown to expressly agglutinate Streptococcus mutants and the other is a protein found in dentin and dental root which mitigates the binding of lactobacillus spp. in this locale. The receptor specific proteins equip the united agent with 1) surgically precise specificity for binding only to the offending bacteria and 2) enhanced residence time of lethal operation for the suicidal linkup. A "cocktail" of combined bactericide-receptor-specific ligands is to be mixed proportionally according to results of outlined experiments designed to elicit the capabilities of the agents to: target or precisely bind only to the cariogenic bacteria; reside for a critical exposure period at the site of action, inhibit glycolysis of bacteria growing on a solid support; and kill bacteria residing on tooth-like hydroxyapatite. PROPOSED COMMERCIAL APPLICATION: The mouthrinse market is $2 billion in size. Except for one prescription brand, no one is effective in preventing or reducing the risk of dental caries.