DEVELOP 3SSH/SAGE TECHNIQUE FOR GENE IDENTIFICATION

描述：（申请人的描述）我们提出开发一种技术， 用3 'cDNA SSH/SAGE鉴定差异表达的 通过修饰和整合关键基因， 差异显示（DD）元素，抑制性减影 基因表达系列分析（SAGE） 技术. 该技术集中于所有的3 '序列， 在给定的细胞群体中表达的基因，去除了大部分丰富的 模板和同源模板的一些部分，并提供了序列 标记每个表达基因以用于数据库搜索。 的差异 两个群体之间表达的基因将通过比较 产生的基因表达谱。 这项技术需要 有限的初始材料，大大降低了基因的难度 鉴定过程中，直接使用EST数据库进行基因 识别，从而提供了一个简单的工具，比较分析 在基因组水平上的基因表达。

DESCRIPTION: (Applicant's Description) We propose to develop a technique we call 3' cDNA SSH/SAGE for the identification of differentially expressed genes at the genomic level through modification and integration of key elements from differential display (DD), suppressive subtraction hybridization (SSH) and serial analysis of gene expression (SAGE) techniques. This technique focuses on the 3' sequence of all of the expressed genes in a given cell population, removes most of the abundant templates and some portion of homologous templates, and provides a sequence tag for each expressed gene to use in a database search. The differentially expressed genes between two populations will be identified by comparison of the resulting gene expression profiles. This technique should require limited initial materials, significantly decrease the difficulty of the gene identification process, directly use the EST database for gene identification, and thus provides a simple tool for the comparative analysis of gene expression at the genomic level.