IDENTIFICATION OF TRANSLATIONALLY CONTROLLED GENES

描述：(改编自申请者摘要)基因调控 翻译水平的表达对许多生物来说很重要 确定和鉴定这些信使核糖核酸的一般程序 需要在翻译控制下的物种。在这个项目中， 胞质中的mRNAs将被分为翻译和非翻译两种 蔗糖梯度离心法。然后放射性的cdna拷贝将被 从这些mRNA物种中制备并用于探测高密度cdna阵列 为了识别那些被抑制或被激活的mRNAs 翻译过来的。然后，将在定义 处于翻译控制下的基因星座 成纤维细胞系中的生长信号。

DESCRIPTION: (Adapted from the Applicant's Abstract) Regulation of gene expression at the translation level is important for many biological processes General procedures for identifying and characterizing those mRNA species that are under translational control are needed. In this project, cytosolic mRNAs will be separated into translated and untranslated species by sucrose gradient centrifugation. Radioactive cDNA copies will then be prepared from these mRNA species and used to probe high density cDNA arrays in order to identify those mRNAs that are repressed or are actively being translated. This procedure will then be tested in defining the constellation of genes that are under translational control in response to growth signals in a fibroblast cell line.