DIDEOXY DNA SEQUENCING WITH TOF-MS

描述(改编自《调查者摘要》)：兴趣持续 找到更快、更便宜的DNA测序方法。其中一个 几个实验室正在研究的方法是形成双脱氧测序 并用质谱仪进行测量。已经取得了一些成功 用MALDI-MS测量短梯子，但不能测量长梯，所以 后者正在进行电泳法测序。不幸的是， 用于此目的的电泳法是一种缓慢且昂贵的技术。在… 至少在一定程度上，DNA分子的碎片化、脱嘌呤和 MALDI-MS中形成的加合离子限制了该方法的性能。在……里面 这一建议提出了一种新的技术，它可能会克服 这些问题，从而提高了质谱学在 双脱氧DNA测序梯形图的测定。

DESCRIPTION (Adapted from the Investigator's Abstract): Interest continues in finding faster and less expensive ways for sequencing DNA. One of the approaches under study in several laboratories is to form dideoxy sequencing ladders and measure them by mass spectrometry. Some success has been achieved in measuring short ladders by MALDI-MS, but not long ladders, so the latter are being sequenced by electrophoresis. Unfortunately, electrophoresis for this purpose is a slow and expensive technique. At least in part, the tendency of the DNA molecule to fragment, depurinate and form adduct ions in MALDI-MS has limited the performance of this method. In this proposal, a novel technique is presented which potentially may overcome these problems, and thereby advance the usefulness of mass spectrometry for the measurement of dideoxy DNA sequencing ladders.