PROTEIN CODING GENES IN THE HUMAN GENOME

一项对整个人类基因组进行排序的重大努力正在进行中。 用计算工具鉴定功能序列元件 变得越来越重要 我们的长期目标是利用 用数学和统计学方法鉴定人类蛋白质编码 人类基因组中的基因，这意味着要找到它们的近似位置， 由启动子和多聚腺苷酸化信号结合的位置， 根据外显子、内含子和编码来描述它们的组织 序列，并通过检查它们的结构/功能来推断它们的结构/功能。 控制/调节元件及其编码的蛋白。 我们有 这一初步赠款有四个具体目标：1）建立和 维护不同类型的人类外显子的质量数据库， 5'和3'外显子及其侧翼序列的单独数据库 来自人类、小鼠、芽殖酵母和裂殖酵母基因。 2)到 扩展我们的内部编码外显子查找器MZER， 编码区域预测 3)开发计算方法， 启动子和第一外显子识别。 4)来开发低分辨率的 蛋白质编码基因的近似定位方法 大规模人类基因组。 的方法和工具 该项目的结果将有助于分子生物学家找到人类 基因和解释结构/功能更快， 准确地

A grand effort is underway to sequence the entire human genome. Identification of functional sequence elements by computational tools has become increasingly important. Our long term goal is to use mathematical and statistical methods to identify human protein coding genes in the human genome, which means to locate their approximate positions bound by the promoters and the polyadenylationsignals, to delineate their organization in terms of their exons, introns and coding sequences, and to infer their structure/function by examining their control/regulatory elements and their encoded proteins. We have the folloiwng four specific aims for this initial grant: 1) To build and to maintain a quality database of human exons of different types, and a separate database of 5' and 3' exons with their flanking sequences from human, mouse, budding yeast and fission yeast genes. 2) To extend our internal coding exon finder MZER to include complete coding region prediction. 3) To develop computational methods for promoter andfirst exon recognition. 4) To develop low-resolution methods for approximate localization of protein coding genes in human genome on large (chromosomal) scale. The methods and tools resulting from this project will help molecular biologists to find human genes and to interpret the structure/functions more quickly and accurately.