DEVELOPMENT OF NOVEL MULTIPLEX TECHNOLOGY FOR GENOMICS

描述：（申请人的摘要）我们提出开发一种新颖的， 成本效益高，高通量的多重技术，基于 飞行时间质谱作为分析工具，可以广泛地 应用于基因组分析领域。 我们打算证明， 验证该技术在四个领域的效用：基因表达， 基因发现、mRNA大小测定和多态性检测。 我们将开发一个 用于监测mRNA表达水平的高通量液相测定。 我们将开发一种高度并行化的方法来识别所有的cDNA 在给定的cDNA文库中，并用于快速鉴定新的cDNA。 我们 将开发一种多重方法来精确测定单个mRNA的大小。 我们将开发一种高通量和低成本的多重检测方法 用于检测单核苷酸多态性。

DESCRIPTION: (Applicant's abstract) We propose to develop a novel, cost-efficient, high-throughput multiplex technology, based on time-of-flight mass spectrometry as the analysis tool, which can be broadly applied in the area of genomic analysis. We intend to demonstrate and validate the utility of this technology in four areas: gene expression, gene discovery, mRNA sizing and polymorphism detection. We will develop a high-throughput, solution-phase assay for monitoring mRNA expression levels. We will develop a highly parallelized method for identifying all cDNAs within a given cDNA library and for fast identification of novel cDNAs. We will develop a multiplexed approach to accurately sizing individual mRNAs. And we will develop a high throughput and cost effective multiplexed assay for the detection of single nucleotide polymorphisms.