MOLECULAR SEQUENCE DATA

描述(改编自《调查者摘要》)：积累 分子序列数据正在以前所未有的速度进行。下一阶段 分子生物学的研究将越来越多地被刻画， 对这些数据进行分类和分析，目的是了解分子 序列信息及其在生物系统中的意义。这个 研究人员的提议旨在加深对基因组的了解 使用经验性、描述性和互动性的结构、功能和进化 统计和计算方法。他们主要关注三个方面 相互关联的领域：一、密码子使用模式分析。详细了解 密码子和残基的选择有助于基因预测，有助于表征 给定基因的属性，以及定义基因类别。他们提出了一项广泛的 中国人群中单个基因和基因类别密码子使用偏向分析 完整的原核生物和真核生物基因组。特别是，调查人员的 研究将涉及不同基因类别的密码子偏好，包括 (I)以功能和/或细胞定位为特征的基因类别； (Ii)由基因大小决定的类别；。(Iii)基因的密码子，分为三个。 部分：氨基1/3部分、中间1/3部分和羧基1/3部分； (Iv)由领先链和滞后链编码的基因；及。(V) 以密码子偏向为特征的水平转移基因 极端情况。二、异常基因的研究，包括外源基因 表达的基因，以及致病岛上的基因。在完整的基因组中或在 扩展的重叠群具有极大的生物学和医学价值 外源基因(例如，横向转移)，或外源基因簇(例如， 致病性或专化岛)，或高表达基因。 基因组序列分析的统计方法。这些措施包括： (A)生物体内和生物间基因组异质性的特征 (例如，在稀有和频繁的核苷酸、基序或 成分偏差)；(B)r-扫描统计的扩展，其评估 标记沿序列分布的异常；和(C)统计 基因组之间的循环序列，其特征是重复家族的数量， 根据它们的大小(BP或AA)、重复序列之间的间距以及 重复家族(基因间、编码、直接、倒置、混合)。

DESCRIPTION (Adapted from the Investigator's Abstract): The accumulation of molecular sequence data is proceeding at an unprecedented pace. The next phase of molecular biology will be increasingly dominated by efforts to characterize, categorize, and analyze these data with the goal of understanding molecular sequence information and its significance in biological systems. The investigators' proposal is aimed at achieving a deeper understanding of genome structure, function, and evolution using empirical, descriptive and interactive statistical and computational methods. They focus primarily on three interrelated areas: I. Analysis of codon usage patterns. Detailed knowledge of codon and residue choices can help in gene prediction, in characterizing properties of a given gene, and in defining gene classes. They propose a broad analysis of codon usage biases for individual genes and gene classes in complete prokaryotic and eukaryotic genomes. In particular, the investigators' studies will concern codon preferences in different gene classes, including (i) gene classes characterized by function and/or cellular localization; (ii) classes determined by gene size; (iii) codons of a gene divided into three parts: the amino 1/3 part, the middle 1/3 part, and the carboxyl 1/3 part; (iv) genes encoded from the leading vs. lagging strand; and (v) classes of horizontally transferred genes characterized with the aid of codon bias extremes. II. Studies of anomalous genes, including alien genes, highly expressed genes, and those in pathogenicity islands. In complete genomes or in extended contigs of great biological and medical interest are characterizations of alien genes (e.g., laterally transferred), or of alien gene clusters (e.g., pathogenicity or specialization islands), or of highly expressed genes. III. Statistical methods for genome sequence analysis. These will include: (a) characterizations of genomic heterogeneity within and between organisms (e.g., in terms of rare and frequent nucleotides, of motifs, or of compositional biases); (b) extensions of r-scan statistics, which assess anomalies in the distribution of markers along sequences; and (c) statistics of recurrent sequences among genomes characterized by numbers of repeat families, by their sizes (bp or aa.), by spacings between repeats, and by properties of repeat families (intergenic, coding, direct, inverted, mixed).