RNA CAPPING IN TRYPANOSOMES

描述(改编自申请人的摘要)：拟议的研究 重点介绍了锥虫体内的RNA封帽反应。调查员已经 束纹夜蛾封顶酶(鸟苷酸转移酶)的纯化 并克隆了丛枝锥虫和锥虫的相应基因。 布鲁塞。拟议研究的长期目标是了解 冠状病毒封端反应的RNA特异性的机理和基础 锥虫。调查员提出了三个具体目标：(1) 对锥虫体封端酶进行结构-功能分析； (2)评估封闭酶基因缺失的后果； 以及(3)搜索在物理上与 封端酶。

DESCRIPTION (Adapted from the Applicant's Abstract): The proposed research focuses on the RNA capping reaction in trypanosomes. The investigator has purified the capping enzyme (guanylyltransferase) from Crithidia fasciculata and has cloned the corresponding genes from C. fasciculata and Trypanosoma brucei. The long-term goal of the proposed research is to understand the mechanism and the basis for the RNA specificity of the capping reaction of trypanosomatids. The investigator proposes three specific aims: (1) to undertake a structure-function analysis of the trypanosome capping enzyme; (2) to assess the consequences of genetic depletion of the capping enzyme; and (3) to search for cellular components that physically interact with the capping enzyme.