Core--Macromolecular crytallography

通过X射线晶体学对大分子结构的分析为理解与癌症相关的生物大分子和组件的有益和不利功能提供了关键数据，这是癌症中心结构生物学计划的研究重点。目前的研究包括广泛的晶体学研究，涉及与癌症相关的基本过程以及预防和治疗。作为普渡癌症中心的一项新举措，高分子晶体学共享资源核心设施的建立将促进癌症相关晶体学研究的强大计划，并刺激新的研究和额外的跨计划合作。该资源的基本服务将是为大分子晶体学提供先进的设施和经验丰富的技术支持，以保持这些设施处于极好的工作状态，供癌症中心的研究人员直接使用。拟议的共享资源整合了结构生物学计划领域的广泛现有共享资产，并为所有癌症中心成员提供访问权限。基本设施包括配备X射线衍射实验和高分子结晶的实验室。关键的衍射仪器包括两台12千瓦的旋转阳极X射线发生器和三台成像板衍射仪，配有聚焦镜光学器件和低温晶体冷却装置。对共享资源的成功同样重要的是非常有经验的支助人员的参与。X射线衍射设备将被纳入这一资源，目前大量和专门用于癌症中心的成员。

The analysis of macromolecular structures by X-ray crystallography provides data critical for an understanding of the beneficial and adverse functions of biological macromolecules and assemblies associated with cancer, which is the focus of research in the Cancer Center's Structural Biology Program. Current studies embrace a wide range of crystallographic research pertaining to basic processes associated with cancer as well as to its prevention and treatment. As a new initiative of the Purdue Cancer Center, the establishment of a Macromolecular Crystallography Shared Resource Core Facility will promote a strong program of cancer-related crystallographic research as well as stimulate new research and additional cross-program collaborations. The essential service of the Resource will be to provide advanced facilities for macromolecular crystallography and experienced technical support to maintain these facilities in superb working condition for direct use by Cancer Center researchers. The proposed shared resource incorporates the extensive existing shared assets of the Structural Biology Program Area and provides access to all Cancer Center members. The essential facilities include laboratories equipped for X-ray diffraction experiments and for the crystallization of macromolecules. Key diffraction instruments include two I2kW rotating anode X-ray generators and three imaging plate diffractometers equipped with focusing mirror optics and cryogenic crystal cooling devices. Equally critical to the success of the shared resource is the involvement of very experienced support personnel. The X-Ray diffraction equipment which will be incorporated into this resource is currently heavily and exclusively used by Cancer Center members.