Structural biology

描述：（申请人描述）结构生物学项目是癌症中心的新成员，是由自然融合而成的。1癌症研究中结构信息的必要性；2 .招募结构生物学家专家组，并在弗吉尼亚大学建立结构生物学中心；这些研究者的许多科学兴趣的癌症焦点，包括多药耐药p -糖蛋白，Ras癌基因，小GTPase， RhoA。磷酸酪氨酸结合适配蛋白Shc。该项目的总体目标是通过跨学科合作，整合肿瘤发生、转移和最终癌症治疗的结构与功能研究。现有的专业知识和方法包括x射线晶体学，核磁共振和EPR光谱，原子力和低温原子力显微镜，全内反射荧光显微镜，激光扫描共聚焦显微镜，x射线制图，能量过滤扫描电子显微镜和电子探针x射线微分析。自项目成立以来（1998年5月），我们在癌症相关蛋白（小GTPase-FAK）的高分辨率结构测定方面取得了重大进展，并启动了多药耐药糖蛋白P的试点项目。该计划还通过国际知名结构生物学家的系列研讨会传播结构方法的价值和使用。

Description: (Applicant's Description) The Structural Biology Program is a new addition to the Cancer Center and resulted from the natural convergence of. 1 The necessity for structural information in cancer research; 2 The recruitment of an expert group of structural biologists and establishment of a Center for Structural Biology at the University of Virginia and; 3 The cancer-focus of many of the scientific interests of these investigators, including the multi-drug resistance P-glycoprotein, the Ras oncogene, the small GTPase, RhoA. and the phosphotyrosine-binding adapter protein, Shc. The overall goal of the Program is to integrate, through interdisciplinary collaborations, structural with functional studies of oncogenesis, metastasis and, ultimately, cancer therapy. Expertise and methodologies available include X-ray crystallography, NMR and EPR spectroscopy, atomic force and cryo-atomic force microscopy, total internal reflectance fluorescence microscopy, laser scanning confocal microscopy, X-ray mapping, energy filtered scanning electron microscopy and electron probe X-ray microanalysis. In the short time since the Program was established (May 1998) we made significant advances in high resolution structure determination of cancer related proteins (small GTPase-FAK), and initiated pilot projects (multi-drug resistance glycoprotein P). The Program also disseminates the value and use of structural methods through a seminar series featuring internationally renowned structural biologists.