IMMUNOBIOLOGY OF INTERLUKIN-12

IL-12在炎症和炎症的调节中发挥重要作用 免疫，作为先天抵抗力和适应性之间的桥梁 豁免权。IL-12的产生在决定 针对感染病原体和肿瘤的保护性Th1反应，而 IL-12的过度产生会导致一系列的病理改变 表现，包括全身炎症反应综合征， 器官特异性自身免疫，结节病，血管病变，而 生产缺陷会导致免疫缺陷和过敏 情况，包括哮喘。分子机制的研究进展 因此，参与IL-12的产生和功能对 对IL-12的生理作用的理解和规划 蛋白质及其基因的临床应用(在感染性疾病中 疾病、肿瘤、严重过敏或作为疫苗接种的佐剂)或 其产生和活性的拮抗剂(在自身免疫中， 结节病等)。为此，我们建议： I.分析IL-12 p40和p35基因的分子机制 表达方式； 分析CD44信号在吞噬细胞中诱导IL-12的作用 细胞及IL-4、IL-13对细胞分化和产生的影响 人单核细胞和树突状细胞对促炎细胞因子的作用 细胞； 三、细胞因子的急性诱导和启动机制分析 IL-12和共刺激诱导产生。

IL-12 is a central player in the regulation of inflammation and immunity, serving as a bridge between innate resistance and adaptive immunity. IL-12 production is important or essential in determining the protective Th1 response against infectious pathogens and tumors, whereas overproduction of IL-12 can result in a series of pathological manifestations, including systemic inflammatory response syndrome, organ-specific autoimmunity, sarcoidosis, vascular lesions, whereas defective production can result in immunodeficiency and allergic situations, including asthma. The study of the molecular mechanisms involved in IL-12 production and function is thus important for the understanding of the physiological role of IL-12 and for the planning of the clinical use of the protein and its genes (in infectious diseases, tumors, severe allergy, or as adjuvants in vaccination) or of antagonists of its production and activity (in autoimmunity, sarcoidosis, etc.). To this end, we propose to: I. Analyze the molecular mechanisms of IL-12 p40 and p35 gene expression; II. Analyze the induction of IL-12 by CD44 signaling in phagocytic cells and the effect of IL-4 and IL-13 on differentiation and production of proinflammatory cytokines by human myelomonocytic and dendritic cells; III. Analyze the mechanisms of acute induction and priming for cytokine production induced by IL-12 and costimulation.