CHARACTERIZATION OF THE RHODOPSIN/TRANSDUCIN INTERFACE

描述(改编自申请人的摘要)：本提案的重点 研究视紫红质/转导蛋白的界面。合成肽和 来自转导蛋白和视紫红质的多肽类似物将被用作 用于高分辨结构研究的特定配体(TRNOE和CPMAS 核磁共振)，当它们与各自的伴侣络合时。通过定义 在光活化过程中相互作用的表面，增加了对 探讨了视紫红质/转导蛋白络合的分子机制。 从三维结构中获得的信息 视紫红质/转导蛋白复合体可以帮助设计治疗方法 与人类相关的疾病(视网膜色素变性和夜盲) 视紫红质的突变。

DESCRIPTION (Adapted from applicant's abstract): The focus of this proposal is to study the rhodopsin/transducin interface. Synthetic peptides and peptide analogs derived from transducin and rhodopsin will be used as specific ligands for high resolution structural studies (TRNOE and CPMAS NMR) when complexed to their respective partners. By defining the interacting surfaces during photoactivation, an increased understanding of the molecular mechanisms of rhodopsin/transducin complexation is sought. The information derived on the three-dimensional structure of the rhodopsin/transducin complex can assist in devising approaches for treating human diseases (retinitis pigmentosa and night blindness) associated with mutations in rhodopsin.