GABA TRANSPORTERS OF RETINAL NEURONS AND GLIA

描述(改编自申请者摘要)：氨基酸GABA发挥作用 脊椎动物在视觉信息处理中的重要作用 视网膜。载体介导的神经元和神经胶质细胞对GABA的转运是关键之一 GABA能通路正常运作的要素。一直以来都是 怀疑中枢GABA的神经元和神经胶质运输系统 神经系统是不同的，具有不同的特性。目的 拟议的项目是检查结构和功能的差异。 视网膜中神经元和神经胶质细胞的GABA转运体之间。 电生理技术，包括全细胞和穿孔贴片 录音，将用来检查GABA转运的特性 酶法分离大鼠脑内水平(神经细胞)和穆勒(胶质)细胞 滑冰视网膜。此外，这些细胞的GABA转运体是 克隆后在卵母细胞中表达；双电极电压钳和巨型 来自卵母细胞的贴片记录将用于检查功能和 药理特性。这些研究应该澄清 神经元和神经胶质细胞GABA转运机制在建立和 维持正常的视网膜功能。

DESCRIPTION (Adapted from applicant's abstract): The amino acid GABA plays an important role in the processing of visual information in the vertebrate retina. Carrier-mediated transport of GABA by neurons and glia is one key element in the proper functioning of GABAergic pathways. It has long been suspected that neuronal and glial transport systems for GABA in the central nervous system are distinct and have differing properties. The purpose of the proposed project is to examine structural and functional differences between neuronal and glial GABA transporters in the retina. Electrophysiological techniques, including whole- cell and perforated patch recordings, will be used to examine the properties of GABA transport in enzymatically isolated horizontal (neural) and Muller (glial) cells of the skate retina. In addition, the GABA transporters of these cells are to be cloned and then expressed in oocytes; dual-electrode voltage-clamp and giant patch recordings from oocytes will be used to examine functional and pharmacological properties. These studies should clarify the role that neuronal and glial GABA transport mechanisms play in establishing and maintaining normal retinal function.