CORE--SCIENTIFIC RESOURCE

科学资源核心负责开展各种 提供数据和组织样本的科学程序 各种项目，并执行全面的数据管理和 统计分析。该核心的具体功能如下 如下：1)以平衡的方式将动物分配给三个人 不同的灌注组以确保年龄的均匀分布 那些认知受损的动物和那些相对 幸免于难。2)之前对所有猴子进行MCI和PET扫描 灌流使定量容量和代谢分析成为可能 被引导了。3)在波士顿进行所有猴子的输液 如果有必要的话，在耶尔克斯。4)准备和分发适当的 每只动物大脑半球的新鲜或冷冻组织样本 所有的项目。5)将整个半球的连续切片从 另一个半球的所有动物和准备四个档案 一系列染色切片(髓鞘、硫宁、HE和AChE)，供 所有的项目。6)进行和监督尸检结果 对所有动物进行尸检，以确保没有隐匿性疾病 这可能会扰乱我们对年龄关系的观察- 大脑的变化与认知功能的变化有关。7)至 维护健康记录和其他数据的综合数据库 Yerkes动物记录系统摘录，实验性 程序执行的操作和结果变量 对不同的项目进行全面的统计分析 这些变量用来确定与年龄相关的主要因素 认知功能障碍。8)鼓励并向其他人提供 研究人员包括我们动物的脑组织和其他组织 这对于这个项目的具体目标来说并不是必需的。

The Scientific Resource core is responsible for carrying out a variety of scientific procedures to provide both data and tissue samples to the various projects and to perform overall data management and statistical analysis. The specific functions of this core are as follows: 1) To assign animals in a balance fashion to the three different perfusion groups to ensure an equal distribution of age animals that are cognitively impaired and those that are relatively spared. 2) To conduct MCI and PET scans on all monkeys before perfusion to enable quantitative volumetric and metabolic analyses to be conducted. 3) To conduct all perfusions of monkeys both in Boston and if necessary at Yerkes. 4) To prepare and distribute appropriate fresh or frozen tissue samples from hemisphere of each animal to all of the projects. 5) To cut serial whole hemisphere sections from the other hemisphere of all of the animals and prepare four archival series of stained sections (myelin, thionin, H&E and AChE) for use by all the projects. 6) To conduct and monitor the results of postmortem autopsies on all animals to ensure that there are no occult diseases that could confound our observations on the relationship of age- related changes in the brain to changes in cognitive function. 7) To maintain an integrated database of health records and other data extract from the Yerkes Animal Records System, experimental manipulations conducted by the program and the outcome variables of the different projects and to perform overall statistical analyses of these variables to identify major factors contributing to age-related cognitive dysfunction. 8) To encourage and make available to other investigators both brain tissue and other tissues from our animals that are not needed for the specific aims of this project.