USING SELF ASSEMBLED MONOLAYERS TO STUDY CELL MIGRATION

描述：这项研究将使用自组装单分子膜(SAM) 金上的烷硫醇作为模型底物用于研究 配体-受体相互作用在细胞迁移中的机制作用。这 工作将集中在成纤维细胞在自组装膜上的迁移上，呈现统一的 RGD三肽的密度(用于细胞黏附)和梯度 碳水化合物N-乙酰氨基葡萄糖；细胞使用半乳糖基转移酶 受体结合固定的碳水化合物并向 增加这种配体的密度。这项工作将使用这些 底物-以及那些呈现非天然配体类似物的底物-到 解决几个机械性问题：(一)确定 成纤维细胞的迁移及其梯度的密度和几何形状 碳水化合物；(2)研究迁移极化的基础 单元格；(3)确定前沿延伸的相对速率 以及移动细胞的后缘的缩回；(Iv)确定 受体的糖基化与迁移的相关性。

DESCRIPTION: This research will use self-assembled monolayers (SAMs) of alkanethiolates on gold as model substrates with which to study the mechanistic roles of ligand-receptor interactions in cell migration. This work will focus on the migration of fibroblasts on SAMs presenting a uniform density of the RGD tripeptide (for adhesion of cells) and a gradient of the carbohydrate N-acetylglucosamine; the cell uses a galactosyltransferase receptor to bind the immobilized carbohydrate and migrate in the direction of increasing density of this ligand. This work will use these substrates--and those presenting non-natural analogs of the ligands--to address several mechanistic issues: (i) determine the relationship between the migration of fibroblasts and the density and geometry of the gradient of carbohydrate; (ii) investigate the basis for polarization of migrating cells; (iii) determine the relative rates for extension of the leading edge and retraction of the trailing edge of the migrating cell; (iv) determine the relevance of glycosylation by the receptor for migration.