TRANSCRIPTIONAL REGULATION OF THE FSH RECEPTOR

描述：（改编自申请人的摘要） 应用是分析FSHR基因的启动子的顺式作用 元件和最终的转录因子相互作用， 激素反应性和该基因的支持细胞特异性表达。 目的1：对FSHR基因近端启动子进行扫描诱变， 将用于鉴定对表达重要的DNA应答元件， 支持细胞系和支持细胞的原代培养物。 在目标2中， FSH、EGF和TGF-β对FSHR基因表达的影响将被 研究了 首先，在原发性肝癌治疗后， 将分析含有FSH、EGF或TGF β的支持细胞培养物。 第二，FSHR启动子对这些相同的转录应答， 物质将通过在Sertoli中的瞬时表达测定来解决 细胞 第三，共表达FSHR启动子构建体与组成型启动子构建体。 参与FSH，EGF， 或TGF β信号转导。

DESCRIPTION: (adapted from the applicant's abstract) The objective of this application is to analyze the promoter for the FSHR gene for cis-acting elements and, ultimately, transcription factors that interact to mediate hormonal responsiveness and Sertoli cell-specific expression of this gene. In aim 1, scanning mutagenesis of the proximal promoter of the FSHR gene will be used to identify DNA response elements important for expression in a Sertoli cell line and primary cultures of Sertoli cells. In aim 2, the effects of FSH, EGF, and TGF-beta on the expression of the FSHR gene will be studied. First, the mRNA encoding the FSHR following treatment of primary cultures of Sertoli cells with either FSH, EGF, or TGFbeta will be analyzed. Second, the transcriptional response of the FSHR promoter to these same substances will be addressed by transient expression assays in Sertoli cells. Third, co-expression of FSHR promoter constructs with constitutively active forms of intracellular kinases that are involved in either FSH, EGF, or TGFbeta signal transduction will be performed.