REGULATION OF THE OVINE FSH BETA GENE BY ESTROGEN

说明(改编自《调查员摘要》)：基于 激活E受体(ER)功能的假说是通过与一个 或更多直接与-105/-72DNA结合的转录因子， 提出了以下目标：目标1寻求定义DNA序列 E对FSHβ的抑制是必需的；Aim 2将识别核 直接与-105到-72bp区域结合的蛋白质，并确定如何 它们与激活的ER相互作用；Aim 3将使用现有的突变体 呃，界定镇压所必需的特征；目标4 包括生产含有野生型或野生型的转基因小鼠 突变的绵羊FSHβ基因以确定 -105/-72序列是体内负调控的关键序列。

DESCRIPTION (Adapted from the Investigator's Abstract): Based on the hypothesis that activated E receptor (ER) functions via association with one or more transcription factors that bind directly to -105/-72 DNA, the following aims are proposed: Aim 1 seeks to define the DNA sequences necessary for repression of FSH beta by E; Aim 2 will identify the nuclear proteins that bind directly to the -105 to -72 bp region and determine how they interact with the activated ER; Aim 3 will use existing mutants of the ER to define the characteristics necessary for repression; and Aim 4 involves the production of transgenic mice that contain either wild-type or mutated ovine FSH beta genes to determine which binding sites in the -105/-72 sequence are critical for negative regulation in vivo.