CLOCK MECHANISM UNDERLYING RHYTHMIC BEHAVIOR

描述(摘自申请者摘要)：这个项目的长期研究目标是用分子术语描述果蝇的昼夜节律振荡器。该实验室以前的研究表明，PER和TIM蛋白在体内相互作用，这种复合体对光的反应是解离的。他们还表明，细胞质PER-TIM复合体的破坏与PER磷酸化和PER核进入的延迟有关。本研究的具体目的是：(1)确定参与PER基因转录后调控的反式作用因子和顺式作用元件；(2)确定PER、TIM和dCLOCK的磷酸化作用，并在体内确定PER和TIM的合作伙伴；(3)表征dCLOCK表达的时空调节，并确定PER和TIM如何与dCLOCK-CyC复合体相互作用。

DESCRIPTION (from applicant's abstract): The long-term research objective of this project is to characterize, in molecular terms, the circadian oscillator of Drosophila. Previous studies from this laboratory have shown that PER and TIM proteins interact in vivo and that this complex disassociates in response to light. They have also shown that the disruption of the cytoplasmic PER-TIM complex is associated with a delay in PER phosphorylation and PER nuclear entry. The specific aims of this current application are to: (1) Characterize trans-acting factors and cis-acting elements that participate in the post-transcriptional control of per mRNA levels, (2) Determine the role of PER, TIM and dCLOCK phosphorylation and identify in vivo partners of PER and TIM, and (3) Characterize the spatial and temporal regulation of dCLOCK expression and determine how PER and TIM interact with the dCLOCK-CYC complex.