TISSUE DIELECTRIC CHANGES DUE TO HYPERTHERMIA

描述：各种形式的癌症治疗，放射治疗，细胞毒性药物， 体温过高等等，都有一个共同的目标。这个目标就是根除 癌细胞。因此，当治疗方法在实验室进行测试时 或应用于临床，他们根除癌症的能力 单元格需要评估。通常，像这样的端点 使用肿瘤生长延迟或肿瘤消退，或者如果是中间的 需要信息，必须采集侵入性组织样本。最近，它 已经确定细胞主要对所有形式的 以两种方式治疗有害刺激；通过程序性细胞死亡 (细胞凋亡)和/或通过坏死。 这项建议的目的是测试电阻抗能力 光谱学(EIS)检测和识别这两种形式的细胞反应 通过非侵入性、活体测量在生长在 裸鼠。使用和不使用维拉帕米的高热治疗将是 用来诱导不同形式的细胞反应。然后是电气 肿瘤的阻抗测量将在以下时间段进行 肿瘤生长迟缓的治疗应用及表现。这些 EIS测量的目的是监测细胞水平的实时反应 在这段时间内肿瘤的发病率。 这项工作将检验假设：1。有可能做出长期的， 可靠、可重复、无创、活体电阻抗 光谱学(EIS)测量组织的电特性。2. EIS能够检测和表征长期、 在其发生过程中高温诱导的坏死反应序列， 以及区分坏死灶的不同特征 不同治疗方法引起的反应不同 肿瘤生长延缓。3.EIS能够检测和表征 高凋亡性实体瘤中的凋亡细胞反应序列 指标，使用非侵入性的活体测量。进展和初步情况 数据证明了用来测量细胞水平变化 环境信息系统。

DESCRIPTION: Cancer therapies of all forms, radiation, cytotoxic drugs, hyperthermia, etc., have one goal in common. That goal is to eradicate cancer cells. Therefore, when therapies are being tested in the laboratory or applied in the clinic, the ability to which they eradicate the cancer cells needs to be evaluated. Typically, endpoints such as tumor-growth-delay or tumor regression are used, or if intermediate information is desired, invasive tissue samples must be taken. Recently, it has been determined that cells predominantly respond to all forms of treatment of deleterious stimuli in two ways; via programmed cell death (apoptosis) and/or via necrosis. The aim of this proposal is to test the capability of Electrical Impedance Spectroscopy (EIS) to detect and identify these two forms of cell response via non-invasive, in vivo measurements in solid human xenografts grown in nude mice. Treatment with hyperthermia, with and without verapamil, will be used to induce the different forms of cell response. Then electrical impedance measurements of the tumors will be made during the period between application of the treatment and manifestation of tumor-growth-delay. These EIS measurements will aim to monitor the real-time cellular-level response of the tumor during this period. The work will test the hypotheses: 1. It is possible to make long-term, reliable, repeatable, non-invasive, in vivo Electrical Impedance Spectroscopy (EIS) measurements of the electrical properties of tissues. 2. EIS is capable of detecting and characterizing long-term, hyperthermia-induced, necrotic response sequences during their occurrence, and of discriminating between different characteristics in the necrotic responses caused by different treatments which result in different tumor-growth-delays. 3. EIS is capable of detecting and characterizing the apoptotic cell response sequence within a solid tumor with high apoptotic index, using non-invasive, in vivo measurements. Progress and preliminary data demonstrate the feasibility of measuring cellular-level change with EIS.