STRUCTURE/FUNCTION COMPARISONS OF HIV 1 & RSV PROTEASES

描述（改编自研究者摘要）：结构 通过RSV和HIV-1晶体学研究获得的信息 蛋白酶将指导本研究试图检查的作用， 决定底物选择的关键残基， 催化效率 尝试将一个PR的特异性改变为 对于异源和同源底物，另一个是 通过定点诱变进行，并通过生物化学 纯化的酶的表征。 PR亚基对称性的影响 最佳的催化效率将通过选择性的 PR同源二聚体的单个亚基的诱变。的信息 从这些实验中收集的信息将被用来构建一个“设计者”， 蛋白酶”作用于HIV-1 RT上正常靶位点以外的其他位点。 最后，将在体内测试改变的蛋白酶，用于进一步的研究。 了解它们在病毒生物过程中的作用 组装和复制。

DESCRIPTION (Adapted from investigator's abstract): The structural information obtained through crystallographic studies on RSV and HIV-1 proteases will guide the present study in attempts to examine the role of critical residues that determine the substrate selection and catalytic efficiency. Attempts to change the specificity of one PR into the other for both heterologous and homologous substrates will be undertaken by site-directed mutagenesis and tested by biochemical characterization of purified enzymes. The effect of PR subunit symmetry for optimal catalytic efficiencies will be examined by selective mutagenesis of individual subunits of the PR homodimer. The information gathered from these experiments would be used to construct a "designer protease" to act on other than normal target sites on HIV-1 RT. Finally, altered proteases will be tested in vivo , for further understanding of their role in biological processes involved in virus assembly and reproduction.