Novel chemoenzymatic synthesis and bioconjugation of peptides and proteins

本项目旨在解决复杂肽胰岛素和相关胰岛素样肽（insulin-like peptides，INSL）及其类似物的生产难题。该项目将使用独特的，可回收的酶，连接较小的肽段与非凡的网站和底物选择性。该项目的预期成果是将这类关键的肽作为生物探针和潜在的治疗剂进行新颖、廉价和可扩展的酶介导工程。

This project aims to solve the critical difficulty of producing the complex peptide insulin and related insulin-like peptides (INSLs) and their analogues. The project will use unique, recyclable enzymes which ligate smaller peptide segments with extraordinary site and substrate selectivity. The expected outcome of the project is novel, cheaper and scalable, enzyme-mediated engineering of this critical class of peptides as biological probes and potential therapeutic agents.