MOLECULAR STUDIES OF LEPTIN ACTION

描述：下丘脑和其他器官， 接受瘦素的信号并将其转化为多型效应物 回应是这项建议的主题。 以下实验 建议：1）研究信号形式的起源和投射， 下丘脑瘦素受体阳性神经元， 这些神经元对瘦素的电生理和转录反应， 和它们的神经递质谱; 2）使用Lox-Cre系统进一步 评估瘦素在CNS和体内其他地方的作用位点;以及3） 研究瘦素的效应反应，通过分析生理 脑室内给予瘦素导致 减少脂肪组织质量。

DESCRIPTION: The mechanism by which the hypothalamus, and other organs, receives leptin's signal and transduce it into a pleiotypic effector response are the subjects of this proposal. The following experiments are proposed: 1) to study the origin and projections of the signaling form of the leptin receptor positive neurons in the hypothalamus, the electrophysiologic and transcriptional responses of these neurons to leptin, and their neurotransmitter profile; 2) to use the Lox-Cre system to further evaluate leptin's sites of action in the CNS and elsewhere in vivo; and 3) to study the effector response to leptin by analyzing the physiologic mechanisms by which intracerebroventricular administration of leptin leads to a reduction in adipose tissue mass.