Mapping Neural Circuits Using Pseudorabie Virus Vectors

使用伪狂犬病病毒载体绘制神经回路

• 批准号: 7339868
• 负责人: JEFFREY M FRIEDMAN
• 金额: $ 35.36万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-15 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7339868
• 关键词: Amygdaloid structure; Animal Viruses; Animals; Antibodies; Bacterial Artificial Chromosomes; Bacterial Genes; Behavior; Binding Proteins; Brain; Brain region; Cells; Complement; Complex; DNA Microarray Chip; DNA Microarray format; Data; Databases; Development; Elements; Enterobacteria phage P1 Cre recombinase; Epitopes; Escherichia; G-Protein-Coupled Receptors; Genes; Genetic; Genetic Transcription; Green Fluorescent Proteins; Hypothalamic structure; Immunoprecipitation; Infection; Label; Localized; Maps; MeSH Thesaurus; Melanocortin 4 Receptor; Melanocyte stimulating hormone; Methods; Motor; Mus; Neural Pathways; Neurons; Nucleoproteins; POMC gene; Partner in relationship; Pathway interactions; Peptides; Peripheral; Play; Population; Pro-Opiomelanocortin; Protein Fragment; Proteins; Publishing; RNA; Recombinants; Reporter Genes; Research Personnel; Salivary Glands; Series; Site; Source; Structure of nucleus infundibularis hypothalami; Suid Herpesvirus 1; System; Testing; Time; Tongue; Tracer; Transgenic Mice; Transgenic Organisms; Variant; Virus; Virus Diseases; Wheat Germ Agglutinins; beta-Galactosidase; cell type; day; enzyme activity; feeding; interest; leptin receptor; neural circuit; neuropeptide Y; novel; programs; promoter; protein expression; recombinase; red fluorescent protein; retrograde transport; vector

DESCRIPTION (provided by applicant): This proposal outlines the development of novel methods for the mapping of complex neural pathways and the specification of the neurons that compose these pathways. Our groups have recently made a recombinant Pseudorabies virus (PRV) that is conditional on cre-recombinase for viability and expression of a GFP reporter gene. This virus has been used successfully to map inputs to specific neuronal populations in defined brain regions. For simplicity we will refer to this and related methods as PAM, Pseudorabies virus assisted mapping. In this application, we propose to further develop PAM so as to allow: 1) Simultaneous Retrograde Tracing from Different Neurons in the Same Brain Region, 2) Simultaneous Retrograde Tracing from Similar Neurons in Different Brain Regions, 3) Anterograde tracing 4) Identification of Neurons Projecting to More than One Site 5) Specification of the neurons that are identified in these mapping studies. To establish the validity of the approach, these studies will be performed in analyses of hypothalamic circuits that regulate feeding. Once the methods are validated, the viruses and animals will be made freely available to other investigators interested in analogous studies of other neuronal pathways.

描述(由申请人提供)：这项提案概述了复杂神经通路映射的新方法的发展，以及组成这些通路的神经元的规范。我们的团队最近制造了一种重组伪狂犬病病毒(PRV)，它以重组酶为条件，以GFP报告基因的活性和表达为条件。这种病毒已被成功地用于将输入映射到特定大脑区域中的特定神经元群体。为简单起见，我们将这种方法和相关方法称为PAM，伪狂犬病病毒辅助作图。在这一应用中，我们建议进一步发展PAM，以允许：1)同一脑区不同神经元的同时逆行追踪，2)不同脑区相似神经元的同时逆行追踪，3)顺行追踪4)投射到多个位置的神经元的识别5)这些标测研究中识别的神经元的规格。为了确定该方法的有效性，这些研究将在调节摄食的下丘脑回路的分析中进行。一旦方法得到验证，病毒和动物将免费提供给其他对其他神经元通路进行类似研究的研究人员。