METABOLIC ADAPTATIONS TO REPETITIVE MYOCARDIAL ISCHEMIA

描述：(改编自调查者摘要)诱导的预适应 通过反复短暂的心肌缺血，每一次都被另一个短暂的 再灌注对随后的缺血再灌注心肌的保护作用 受伤。预适应的有益后果之一是渐进性的。 高能磷酸盐化合物的保存，这反映在 间质液体(ISF)增加的逐渐增强的衰减 嘌呤代谢物。拟议的研究将审查 通过解决两个特定目标来减少嘌呤代谢物的积累：1) 通过确定诱导所需的最短时间和缺血程度 在随后的全脑缺血期间减少嘌呤代谢产物的积聚 2)通过测定腺苷激酶或腺苷脱氨酶的作用 抑制ISF嘌呤代谢产物蓄积的实验研究 在预适应过程中腺苷的形成。

DESCRIPTION: (Adapted from the Investigator's Abstract) Preconditioning induced by repeated brief myocardial ischemia each separated by another brief period of reperfusion protects the myocardium from subsequent ischemia reperfusion injury. One of beneficial consequences of preconditioning is progressive preservation of high energy phosphate compounds which is reflected by the progressively greater attenuation of the increase in interstitial fluid (ISF) purine metabolites. The proposed research will examine the threshold of the attenuated purine metabolite accumulation by addressing two Specific Aims: 1) by determining the minimum time and degree of ischemia necessary to induce attenuated purine metabolite accumulation during subsequent global ischemia and 2) by determining the effect of adenosine kinase or adenosine deaminase inhibition on ISF purine metabolite accumulation and by studying the rate of adenosine formation during preconditioning.