MECHANISMS OF ACTIN DEPENDENT ORGANELLE MOTILITY

描述（改编自研究者摘要）： 建议的研究是表征肌动蛋白依赖性色素的机制， 硬骨鱼RPE细胞颗粒运动的研究一个模型提出向心力 色素颗粒的聚集与肌动蛋白的向心流动有关 而颜料颗粒的离心分散受到沿着 肌动蛋白丝通过肌球蛋白马达，将被测试。具体目标是：（1） 表征RPE细胞中F肌动蛋白的超微结构组织，2） 通过S1确定RPE顶端投射中肌动蛋白丝的极性 标记，3）确定颗粒是否附着在RPE细胞表面 进行向心运输和4）在RPE细胞中定位肌球蛋白VI和VIIa。

DESCRIPTION (adapted from investigator's abstract): The objective of the proposed research is to characterize the mechanism of actin-dependent pigment granule motility in RPE cells of teleosts. A model proposing that centripetal aggregation of pigment granules is linked to centripetal flow of actin filaments while centrifugal dispersion of pigment granules is affected along actin filaments via myosin motors, will be tested. Specific aims are to 1) characterize the ultrastuctural organization of F actin in RPE cells, 2) determine the polarity of actin filaments in RPE apical projections via S1 labeling, 3) determine whether particles attached to the surface of RPE cells undergo centripetal transport and 4) localize myosins VI and VIIa in RPE cells.