EFFECT OF GROWTH HORMONE ON CRANIOFACIAL PROPORTIONS

这项研究调查了生长激素(GH)变化的影响 面部生长，牙齿大小和发育，以及咬合。颅面 比例变化，局部的骨沉积和吸收模式， 牙齿的大小、形状、发育和咬合情况将在三年内进行检查 内分泌生长障碍的主要模型：1)巨型转基因(MTrGH) 小鼠；2)Snell侏儒(dw/dw)突变小鼠；以及3)GH注射的绒猴 猴子。一般说来，这项工作要检验的假设是 在所有三个试验组的大小和形状上的变化 颅面复合体将由不同的伸展引起(在 转基因小鼠、绒猴)或截断(在矮小鼠中) 在各自的对照中观察到的异速生长(相对)模式 组。此外，在头面部比例上的差异 实验和对照假设是由不同的 骨吸收和骨沉积的一般速率，而不是基本速率 这些基本区域模式本身的变化，以及大小、形状和 牙齿的萌出和咬合模式被假设为 试验组和控制组的情况相似。这些假设将是 使用从以下位置获取的线性尺寸和坐标点进行测试 头骨和X光片。骨的扫描电子显微镜检查 表面将决定吸收和再吸收模式的分布 生长过程中的沉积。 我的职业目标是继续我的初步研究 生长激素对骨骼生长发育模式的影响 比例，并将这些更充分地扩展到试验性和 临床领域。RCDA将特别推动我的研究事业 极大地减轻了我异常沉重的教学负担，这是一个障碍 为有效实施涉及的和长期的实验室和 临床调查。此外，这一奖项将使我能够开发新的 我在骨骼生长和骨发育研究中的研究方向 比例，特别是生长的纵向和实验研究 激素在灵长类动物模型中的应用，以及扫描电子显微镜分析在 股骨近端骨沉积和吸收类型的测定 正常和异常生长过程中的头面部复合体。 如果授予RCDA，该机构的支持计划包括减少 我的正常教学负担减少了一半以上，这样分配的总时间 因为所描述的研究将是80%，而我的时间不超过20% 用于相关的教学和咨询活动。此外，还可以访问 具体的研究资源，包括设备和培训 生长激素(GH)给药实验及血药浓度测定 生长激素水平将由我的家庭部门和其他部门提供 就在西北大学。

This research investigates the effects of variation in growth hormone (GH) on facial growth,dental size and development, and occlusion. Craniofacial proportion changes, localized patterns of bone deposition and resorption, and tooth size, shape, development and occlusion will be examined in three primary models of endocrine growth disturbance: 1) giant transgenic (MTrGH) mice; 2) Snell dwarf (dw/dw) mutant mice; and 3) GH-administered marmoset monkeys. Generally stated, the hypothesis to be tested in this work is that in all three experimental groups changes in the size and shape of the craniofacial complex will result from differential extension (in the transgenic mice, marmosets) or truncation (in the dwarf mice) of the patterns of allometric (relative) growth observed in the respective control groups. In addition, differences in craniofacial proportions between experimentals and controls are hypothesized to result from differences in general rates of bone resorption and deposition, as opposed to fundamental shifts in these basic regional patterns themselves, and the size, shape and patterns of eruption and occlusion of the teeth are hypothesized to be similar in both experimental and control groups. These hypotheses will be tested by utilizing linear dimensions and coordinate points taken from skulls and radiographs. Scanning electron microscopic examination of bone surfaces will determine the distribution of patterns of resorption and deposition during growth. My career objectives are to continue my preliminary studies of the influence of growth hormones on patterns of skeletal growth and proportions, and to extend these more fully into the experimental and clinical realms. An RCDA would particularly advance my research career by dramatically reducing my unusually heavy teaching load which is an obstacle to the effective implementation of involved and prolonged laboratory and clinical investigations. Moreover, this award would allow me to develop new research directions in my investigations of skeletal growth and proportions, particularly longitudinal and experimental studies of growth hormone administration in a primate model, and the use of SEM analysis in the determination of patterns of bone deposition and resorption in the craniofacial complex during normal and abnormal growth. The Institution's support plans if an RCDA is awarded include a reduction of my normal teaching load by more than half, such that total time allotted for the research described would be 80% with no more than 20% of my time spent on related teaching and advising activities. In addition, access to specific research resources including equipment as well as training in growth hormone (GH) administration experiments and the determination of growth hormone levels will be provided by my home departments and others here at Northwestern.