B LYMPHOCYTE ACTIVATION

眼前的目标是描绘出通过什么机制 功能上重要的分子，如Lyb2、Ia和BSF-1 受体参与B淋巴细胞的活化。长期 目标是设计方法来调节高血压病患者的B细胞功能 低免疫状态以及确定的生化基础 B细胞的生长和分化 生物化学、细胞免疫学和分子生物学。 这项研究的长期目标是了解 相关免疫异常的潜在基础 年龄。最初，研究的重点将是功能角色 两个B细胞表面分子Lyb2和Ia。战略将是 开发的目的是克隆Lyb2抗原的基因。这个概念是一个 独特的B细胞亚群参与抗体反应 将对多糖类抗原进行评估。B的可能性 细胞亚群的生长或成熟因子可能不同。 他们表达的受体将通过制备单克隆来研究 这种B细胞表面分子的抗体。生化和 老年小鼠B细胞的表型异常将是 学习。青年和成人B细胞谱系的差异 衰老小鼠将通过量化表达来确定 核型小鼠重链可变区基因家族 酸杂交技术。 这些研究应该使我们能够更好地了解B细胞的生长 和差异化，并制定更好的战略 免疫调节。

The immediate goals are to delineate the mechanisms by which functionally important molecules such as Lyb2, Ia and BSF-1 receptors participate in B lymphocyte activation. Long term goals are to devise ways to regulate B cell function in hyper- and hypo-immune states as well as to define the biochemical basis of B cell growth and differentiation using the approaches of biochemistry, cellular immunology and molecular biology. The long term objectives of this research are to understand the underlying basis for the immune abnormalities associated with age. Initially, the focus of the research will be the functional role of two B cell surface molecules Lyb2 and Ia. Strategies will be developed to clone the gene for Lyb2 antigen. The concept that a unique B cell subset is involved in antibody responses to polysaccharide antigens will be evaluated. The possibility that B cell subsets may differ in the growth or maturation factor receptors they express will be studied by preparing monoclonal antibodies to such B cell surface molecules. Biochemical and phenotypic abnormalities in B cells from aged mice will be studied. The repertoire differences in B cells from young and aged mice will be determined by quantitating the expression of the murine heavy chain variable region gene families by nucleic acid hybridization techniques. These studies should enable us to better understand B cell growth and differentiation and to develop better strategies for immunomodulation.