ENDOCRINE RESPONSIVENESS IN HETEROTHERMY

候选人计划留在阿拉斯加大学继续深造 关于他独特的动物模型--冬眠的北极地松鼠的研究 帕里氏嗜精菌。获奖将使他能够增加自己的时间 可用于研究的比例约为65%至85%。因为 抗冻性研究涉及到的技术和方法 应聘者未接受过专门培训的，收到RCDA 会特别有利，让他有时间与 其他机构的低温生物学专家。北极研究所 生物学完全支持候选人的工作，并提供了一个独特的 在环境和环境方面进行研究的合适环境 设施；相对地理隔绝和面积较小的 然而，研究所限制了互动的潜力，除非旅行 通过限制非研究责任而成为可能。 这项拟议的研究调查了(I) 生殖内分泌系统与对季节变化身体的控制 冬眠哺乳动物的温度(Tb‘s)，以及(Ii)S. Parryii可以在不冷冻的情况下采用零度结核病。与以下目标相关的目标 生殖功能和冬眠包括：(1)测量血浆 冬眠结束时生殖激素水平；(2)中枢神经系统检测 冬眠对雄激素抑制的敏感性；ND(3)确定是否 血浆类固醇激素结合蛋白可防止这种抑制 冬眠。与抗冻有关的目标包括：(1)确定 对零度以下耐受性和抗冻动物血液检测的限制 用于防冻剂和冷冻保护剂；(2)测量血液的季节性变化 过冷点、血清蛋白和脂蛋白；(3)鉴定 血液中的冰核因子和确定是否有沙鼠 消除这些，以避免结冰；(4)人工接种冰冻 研究冷冻防御机制的过冷动物；和(5) 考察非抖动生热作用在稳定零下温度中的作用 体温过低。 这些实验应该阐明新的功能关系 生殖内分泌系统和中枢神经系统对结核病的控制。他们的地址是 生殖卷土重来的问题是如何在 黑暗和周期性的低体温冬眠。其他 实验调查了表面上独特的机制的基本问题 哺乳动物的耐寒性，其答案可能与 移植器官的冷冻保存和对严重低温的耐受性。

The candidate plans to remain at the University of Alaska to pursue research on his unique animal model, the hibernating arctic ground squirrel Spermophilus parryii. The award would enable him to increase his time available for research from approximately 65 to 85%. Because the investigation of freeze resistance involves techniques and approaches in which the candidate has not been specifically trained, receipt of an RCDA would be especially advantageous in allowing him time to interact with experts in cryobiology at other institutions. The Institute of Arctic Biology fully supports the candidate's work and offers a uniquely appropriate situation for the studies in terms of environment and facilities; the relative geographical isolation and small size of the Institute, however, limit the potential for interactions unless travel is made possible by limiting non-research responsibilities. The proposed research investigates (I) interrelationships between the reproductive endocrine system and control over seasonally changing body temperatures (Tb's) in hibernating mammals, and (II) mechanisms by which S. parryii can adopt subzero Tb's without freezing. Aims related to reproductive function and hibernation include: (1) measuring plasma reproductive hormone levels at the end of hibernation; (2) testing for CNS sensitivity to androgen inhibition of hibernation; nd (3) determining if plasma steroid hormone-binding proteins prevent such inhibition during hibernation. Aims related to freeze resistance include: (1) determining limits to subzero tolerance and testing blood from freeze-resisting animals for antifreeze and cryoprotectants; (2) measuring seasonal changes in blood supercooling points and serum proteins and lipoproteins; (3) identifying ice nucleation factors in blood and determining if ground squirrels eliminate these to avoid freezing; (4) artificially inoculating freezing in supercooled animals to investigate mechanisms of freeze defense; and (5) examining the role of nonshivering thermogenesis in stabilizing subzero hypothermia. These experiments should elucidate novel functional relationships between the reproductive endocrine system and CNS control over Tb. They address the question of how reproductive recrudescence begins during the constant darkness and periodic low body temperatures of hibernation. Other experiments investigate basic questions about apparently unique mechanisms of freeze resistance in mammals, answers to which may relate to cryopreservation of transplant organs and tolerance to severe hypothermia.