LAMININ AND FIBRONECTIN

这项RCDA建议的长期研究目标是了解 细胞外基质(ECM)分子在周围神经中的作用 系统(PNS)开发。细胞外基质分子调节细胞的形状、运动和 胚胎发育过程中的分化和粘附性糖蛋白 纤维连接蛋白(FN)和层粘连蛋白(LAM)在空间和时间上均有分布 调节神经脊分化和神经纤维的某些方面 伸长率。三个具体目标定义了解决这个问题的方法。 首先，FN和LAM的细胞结合域与 细胞与完整分子的相互作用？组织培养研究将 探针1)黏附在细胞与每个结构域相互作用中的作用，2) 活细胞与特定区域相互作用时的行为，以及3) 细胞骨架与这些相互作用相关。第二，FN和LAM怎么样？ 被发育中的PNS中的细胞所使用？方法将是 光镜和电子显微镜水平的免疫细胞化学， 分析细胞与复杂基质的体外相互作用，以及 体内细胞-细胞外基质相互作用的扰动。第三，角色是什么？ FN和LAM的明确区域在迁移和分化中的作用 神经脊细胞？ 有了RCDA的资金，P.I.将扩展到神经脊的方法 差异化，但学到的技术将适用于研究 神经元的行为也是如此。延长的时间段将花在 10伦敦国王学院杰雷米·布罗克斯博士的实验室 利用重组DNA技术进行亚群分化的研究 胚胎和再生细胞，以及2)神经科学研究所 大学。俄勒冈州(尤金)的几个实验室正在研究神经质 斑马鱼的系统开发使用广泛的技术(例如。 分子生物学、激光消融活动物细胞、单克隆化 抗体产生)。 随着时间从教学和委员会的职责中解脱出来，P.I. 也将能够在以下方面寻求更有效的研究机会 大学。新墨西哥州。特别是，超乎寻常的图像分析 UNM的设施将得到利用。这一次也将允许私家侦探。 开发发育神经生物学的研究生课程，与 意在吸引学生从事这一领域的研究。

The long-range research goal of this RCDA proposal is to understand the roles of extracellular matrix (ECM) molecules in peripheral nervous system (PNS) development. ECM molecules regulate cell shape, movement and differentiation during embryogenesis, and the adhesive glycoproteins fibronectin (FN) and laminin (LAM) are located spatially and temporally to mediate aspects of neural crest differentiation and nerve fiber elongation. Three Specific Aims define approaches to this question. First, what is the relevance of the cell binding domains of FN and LAM to cell interaction with the intact molecule? Tissue culture studies will probe 1) roles of adhesion in cell interaction with each domain, 2) behavior of living cells as they interact with specific domains, and 3) cytoskeletal correlates of these interactions. Second, how are FN and LAM used by cells in the developing PNS? Approaches will be immunocytochemistry at the light and electron microscopic levels, analysis of cell interaction with complex matrices in vitro, and perturbation of cell-ECM interactions in vivo. Third, what are the roles of defined regions of FN and LAM in migration and differentiation of neural crest cells? With RCDA funding, the P.I. will extend approaches to neural crest differentiation, but the techniques learned will be applicable to studies of neuronal behavior as well. Extended periods of time will be spent in 10 the lab of Dr. Jeremey Brockes, Kings College London, to study recombinant DNA approaches to differentiation of subpopulations of embryonic and regenerating cells, and 2) the Neuroscience Institute at the Univ. of Oregon (Eugene) where several labs are studying nervous system development in zebrafish using a wide range of techniques (eg. molecular biology, lasar ablation of cells in living animals, monoclonal antibody production). With time release from teaching and committee responsibilities, the P.I. also will be able to pursue more effectively research opportunities at the Univ. of New Mexico. In particular, the exceptional image analysis facilities at UNM will be exploited. This time will also permit the P.I. to develop a graduate course in developmental neurobiology, with the intention of attracting students to this area of research.