FORMATION AND ELIMINATION OF SYNAPSES

为了了解神经系统是如何运作的， 突触的结构、功能和形成。 一个最 突触上的一个重要结构是递质释放的部位： 活动区 在此建议的诱导活性zoe在异位 将用细胞内记录研究青蛙的终板部位， 冷冻断裂电子显微镜 异位连接将通过去除原始终板重新诱导 将原始神经植入无终板区域。 是否 将检查在异位接合处形成的活性区， 特别是与后接合褶皱有关，如果后者也 形成了 结果将与以前的再生研究进行比较 原始终板的活动区 此外， 将分析异位连接处的活动区，以研究是否 与发射器释放的增加相关， 细胞内记录 拟议的研究将阐明如何独特的组织， 诱导分化活性区。 它还将提供一个更好的 对活动带构造与发射体相关性的认识 释放功效。 关于突触如何形成和工作的基本知识 将有助于理解某些神经系统疾病的机制 可能涉及递质释放不足的疾病。

To understand how the nervous system operates it is important to study structure, function and formation of the synapse. One of the most important structure at the synapse is the site of transmitter release: the active zone. In this proposal the induction of the active zoe at ectopic endplate sites in the frog will be studied with intracellular recording and freeze-fracture electron microscopy. Ectopic junctions will be induced de novo by removing the original endplate zone and implanting original nerves to endplate-free areas. Whether or not the active zone is formed at the ectopic junction will be examined, especially in relation to the postjunctional fold if the latter is also formed. The result will be compared with a previous study on regeneration of the active zone at the original endplate. In addition the morphometry of active zones at ectopic junctions will be analyzed to study if it correlates with the increase in transmitter release shown with intracellular recording. The proposed research will elucidate how the unique organization of the active zone is induced and differentiated. It will also provide a better understanding on correlation between active zone structures and transmitter released efficacy. This basic knowledge on how synapses form and work would throw light on understanding the mechanisms of certain neurological diseases which may involve deficiency in transmitter release.