RENAL RESPONSES TO CHANGES IN PROSPHATE SUPPLY

Scheinman博士是一名肾病学家，他的教职生涯始于助理 纽约州立大学北部医学中心的医学教授。他收到了他的 耶鲁大学内科医学博士学位和住院医师培训，以及 在北州立大学和耶鲁大学进行的肾病学专科培训。在他的 研究训练他使分离的灌流大鼠肾脏用作 甲状旁腺激素效应的体外模型。他的目标是建立 他是一名主要的独立实验室调查员，他建议 对监管中一个重大悬而未决问题的初步研究 无机磷(PI)动态平衡：信号改变的本质 体内PI供应变化过程中肾脏对PI的重吸收。建议数 研究将检查(1)PI是否能直接影响肾脏PI 运输；(2)胞外或摄食pi与 肾细胞内PI；和(3)肾外信号的存在 控制肾脏的PI重吸收。我们将考察PI的直接作用 用一种新的模型研究肾转运的调节：大鼠 肾脏将在体外灌流不同成分的介质，以及 BBMV将由这些肾脏制成并进行研究。细胞内PI将是 在灌流的肾脏中进行评估，可能还有活体肾脏，使用新的 31P-核磁共振波谱技术。最后，我们将开发一个模型。 肠外营养期间肾脏对PI供应变化的适应 这一模型将被用来确定 胃肠道在这种肾脏适应中的作用。这类研究可能 最终阐明了PI动态平衡是 紊乱，可能包括维生素D抵抗的低磷血症 软骨病、肾功能不全和某些形式的肾结石疾病。 欧文·M·韦纳博士将担任赞助商，乔治·C·利维博士将担任赞助商 次级赞助商将指导核磁共振研究。通过利维博士， Scheinman将有权使用N.I.H.资源进行多核核磁共振和 数据处理位于锡拉丘兹大学。玛丽·埃伦·特里布尔博士 将合作进行BBMV研究，迈克尔·马吉德博士将 在涉及父母营养的研究上进行合作。此外， 纽约州立大学北部医学中心有许多活跃在肾脏生理学领域的科学家 以及跨学科咨询的悠久传统。

Dr. Scheinman is a nephrologist beginning his faculty career as Assistant Professor of Medicine at S.U.N.Y.-Upstate Medical Center. He received his M.D. degree and residency training in Internal Medicine at Yale, and nephrology subspecialty training at both Upstate and Yale. During his research training he adapted the isolated perfused rat kidney for use as an in vitro model of parathyroid hormone effects. His goal is to establish himself as a major independent laboratory investigator, and he proposes initially to study a substantial unsolved problem in the regulation of inorganic phosphate (Pi) homeostasis: the nature of the signal that alters renal Pi reabsorption during changes in body Pi supply. The proposed studies will examine (1) whether Pi can directly influence renal Pi transport; (2) the relationship between extracellular or dietary pi and renal intracellular Pi; and (3) the existence of an extrarenal signal governing renal Pi reabsorption. A direct role of Pi will be examined using a new model for studying the regulation of renal transport: rat kidneys will be perfused in vitro with media of various compositions, and BBMV will be made from these kidneys and studied. Intracellular Pi will be assessed in perfused kidneys, and possibly kidneys in vivo, using the new technique of 31P-NMR spectroscopy. Finally, a model will be developed of renal adaptation to changes in Pi supply during parenteral nutrition in rats, and this model will be used to determine the importance of the gastrointestinal tract in this renal adaptation. Such studies may eventually shed light on a variety of conditions in which Pi homeostasis is disordered, possibly including vitamin D-resistant hypophosphatemic rickets, renal insufficiency, and certain forms of renal stone disease. Dr. Irwin M. Weiner will serve as sponsor, and Dr. George C. Levey as secondary sponsor will direct the NMR studies. Through Dr. Levy, Dr. Scheinman will have access to the N.I.H. Resource for Multinuclei NMR and Data Processing located at Syracuse University. Dr. Mary Ellen Trimble will collaborate on the BBMV studies, and Dr. Michael Meguid will collaborate on studies involving parental nutrition. In addition, the SUNY-Upstate Medical Center has many scientists active in renal physiology and a long tradition of interdisciplinary consultation.