NHLBI CLINICAL INVESTIGATOR AWARD PROGRAM

免疫介导的血小板破坏发生在特发性（免疫） 血小板减少性紫癜，过渡期后紫癜，新生儿 同种免疫性血小板减少症（NITP）和其他疾病状态。 的 与这些疾病有关的抗原和抗体并不很好地 表征了 利用我们开发的识别抗原的技术 携带血小板蛋白质，我们将研究的性质和结构， 这些血小板抗原和介导血小板聚集的抗体 杀伤性 该技术涉及血小板的电泳分离 膜蛋白，转移到硝酸纤维素纸上，暴露于抗体， 和使用放射性标记的第二抗体检测抗体结合。 我们 建议进一步表征PLA1血小板抗原，并研究 NITP中抗PLA1抗体的性质。 我们会尝试找出 血小板结合的血小板Fc受体或Fab结合位点 IgG可以结合。 最后，我们将进一步表征抗原， 我们已经在患有ITP的儿童受试者中鉴定了抗体。

Immune mediated platelet destruction occurs in idiopathic (immune) thrombocytopenia purpura (ITP), post transition purpura (PTP), neonatal isoimmune thrombocytopenia (NITP), and in other disease states. The antigens and antibodies involved in these conditions are not well characterized. Using techniques we have developed for identifying antigen bearing proteins of platelets, we will study the nature and structure of these platelet antigens and the antibodies which mediate platelet destruction. The technique involves electrophoretic separation of platelet membrane proteins, transfer to nitrocellulose paper, exposure to antibody, and detection of antibody binding using a radiolabeled second antibody. We propose to further characterize the PLA1 platelet antigen and to study the nature of the anti PLA1 antibodies in NITP. We will attempt to identify the platelet Fc receptor or Fab binding site to which platelet associated IgG may bind. Finally, we will further characterize the antigens and antibodies we have identified in a subject of children with ITP.